Related Experiment Video
Updated: Jul 17, 2025

07:18
An Electroporation Method to Transform Rickettsia spp. with a Fluorescent Protein-Expressing Shuttle Vector in Tick Cell Lines
Published on: October 11, 2022
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Completed genomes for Rickettsia rickettsii isolated from ticks and quality controlled for motility phenotype
Adam M Nock1, Tina R Clark1, Kent Barbian2
1Host-Parasite Interactions Section, Laboratory of Bacteriology, Rocky Mountain Laboratories, NIAID, NIH, Hamilton, Montana, USA.
Microbiology Resource Announcements
|September 1, 2023
Abstract:
Complete genomes of Rickettsia rickettsii were sequenced with Illumina and PacBio technologies from low-passage isolates from ticks. These isolates were quality controlled for intact roaM, a regulator of actin-based motility that is negatively selected for in culture. The Sheila Smith strain was re-sequenced using the same methodology.

