Related Experiment Video

Updated: Jul 17, 2025

Visualizing Mitophagy with Fluorescent Dyes for Mitochondria and Lysosome
07:56

Visualizing Mitophagy with Fluorescent Dyes for Mitochondria and Lysosome

Published on: November 30, 2022

4.6K

BCL2L13 promotes mitophagy through DNM1L-mediated mitochondrial fission in glioblastoma

Jiwei Wang1,2, Anbin Chen3, Zhiwei Xue1,2

  • 1Department of Neurosurgery, Qilu Hospital, Cheeloo College of Medicine and Institute of Brain and Brain-Inspired Science, Shandong University, 250012, Jinan, China.

Cell Death & Disease
|September 2, 2023
PubMed

Insights

Bcl2-like 13 (BCL2L13) promotes Glioblastoma multiforme (GBM) growth by increasing mitochondrial fission and mitophagy. Targeting BCL2L13 offers a potential therapeutic strategy for GBM patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • Glioblastoma multiforme (GBM) requires new diagnostic and therapeutic approaches.
  • BCL2 like 13 (BCL2L13), a BCL2 family member, influences tumor cell growth and apoptosis.
  • The role and mechanism of BCL2L13 in GBM are not well understood.

Purpose of the Study:

  • To investigate the clinical significance, biological role, and mechanism of BCL2L13 in Glioblastoma multiforme.
  • To determine if BCL2L13 expression is altered in GBM and its functional impact.

Main Methods:

  • Analysis of BCL2L13 expression in GBM cell lines and clinical tissues.
  • Investigation of the molecular mechanism involving BCL2L13, DNM1L, mitochondrial fission, and mitophagy.
  • Assessment of the functional role of BCL2L13 in GBM cell proliferation and invasion in vitro and in vivo.

Main Results:

  • BCL2L13 expression was significantly upregulated in GBM cell lines and patient samples.
  • BCL2L13 was found to target DNM1L at Ser616, inducing mitochondrial fission and increased mitophagy.
  • Upregulation of BCL2L13 promoted GBM cell proliferation and invasion.

Conclusions:

  • BCL2L13 promotes GBM progression through DNM1L-mediated mitochondrial fission and enhanced mitophagy.
  • BCL2L13 represents a potential molecular target for novel GBM therapies.

More Related Videos

In Vitro and In Vivo Detection of Mitophagy in Human Cells, C. Elegans, and Mice08:40

In Vitro and In Vivo Detection of Mitophagy in Human Cells, C. Elegans, and Mice

Published on: November 22, 2017

17.6K
Sensitive Measurement of Mitophagy by Flow Cytometry Using the pH-dependent Fluorescent Reporter mt-Keima09:13

Sensitive Measurement of Mitophagy by Flow Cytometry Using the pH-dependent Fluorescent Reporter mt-Keima

Published on: August 12, 2018

14.9K

Related Concept Videos

Translocation of Proteins into the Mitochondria01:19

Translocation of Proteins into the Mitochondria

Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
3.1K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K
The Intrinsic Apoptotic Pathway01:31

The Intrinsic Apoptotic Pathway

Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
6.6K
Electron Transport Chain: Complex I and II01:46

Electron Transport Chain: Complex I and II

The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
14.5K
Delivery Pathways to the Lysosome01:36

Delivery Pathways to the Lysosome

Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
6.6K
Autophagic Cell Death01:18

Autophagic Cell Death

Christian de Duve discovered “autophagy,” a process in which cellular components are engulfed by membrane-bound organelles called autophagosomes. The autophagosomes then fuse with lysosomes to digest the enclosed contents. Autophagy is generally activated in cells to prevent cell death. However, cell death is triggered when the damage is beyond repair.
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
3.4K