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Spinal cord injury immunosensor: Sensitive detection of S100β on interdigitated electrode sensor
Hao Zhang1, Subash C B Gopinath2,3,4,5, Yajun Hu6
1Department of Surgery, Hospital of Northwestern Polytechnical University 127 Youyi West Road, Beilin District, Xi 'an 710072, China.
Heliyon
|September 4, 2023
Summary
A novel immunoassay using S100β biomarker on an interdigitated electrode sensor can effectively diagnose spinal cord injury. This sensitive method detects S100β at low levels, aiding in early injury identification and management.
Area of Science:
- Biomedical Engineering
- Neuroscience
- Biomarker Detection
Background:
- Spinal cord injury (SCI) disrupts neural signals, leading to motor and sensory deficits, including paralysis.
- S100β protein is identified as a potential biomarker for diagnosing SCI and its underlying causes.
Purpose of the Study:
- To develop and validate an S100β immunoassay on an interdigitated electrode sensor for SCI diagnosis.
- To optimize antibody immobilization for enhanced sensor performance.
Main Methods:
- Developed an immunoassay utilizing an interdigitated electrode sensor.
- Optimized anti-S100β antibody immobilization using a premixing method with 3-Aminopropyl)triethoxsilane.
- Assessed sensor performance, including limit of detection and specificity through biofouling experiments.
Main Results:
- The optimized antibody immobilization method doubled antibody attachment efficiency.
- The immunoassay demonstrated a limit of detection of 1 pg/mL for S100β.
- High linearity (R² = 0.9907) was observed for S100β concentrations up to 3000 pg/mL.
- Specificity was confirmed by the lack of response to other proteins like GFAP and GABA.
Conclusions:
- The developed S100β immunoassay is a sensitive and specific tool for diagnosing spinal cord injury.
- This biosensor technology offers a promising approach for early and accurate SCI detection.
