Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A dot-blot assay for heparin-binding proteins.

N Hirose, M Krivanek, R L Jackson

    Analytical Biochemistry
    |August 1, 1986
    PubMed
    Summary

    This study presents a new method to detect and quantify low amounts of heparin-binding proteins. The assay uses radioautography and densitometry, offering a sensitive tool for protein analysis.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Caregiving centenarians: Cross-national comparison in Caregiver-Burden between the United States and Japan.

    Aging & mental health·2019
    Same author

    Prophylactic infusion of phenylephrine is effective in attenuating the decrease in regional cerebral blood volume and oxygenation during spinal anesthesia for cesarean section.

    International journal of obstetric anesthesia·2018
    Same author

    Development of a Cavity Disinfectant Containing Antibacterial Monomer MDPB.

    Journal of dental research·2016
    Same author

    The C-terminus of amelogenin enhances osteogenic differentiation of human cementoblast lineage cells.

    Journal of periodontal research·2016
    Same author

    Effects of enzymatic degradation after loading in temporomandibular joint.

    Journal of dental research·2014
    Same author

    Denture wearing during sleep doubles the risk of pneumonia in the very elderly.

    Journal of dental research·2014

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Analytical Chemistry

    Background:

    • Heparin-binding proteins play crucial roles in various biological processes.
    • Accurate detection and quantification of these proteins are essential for research and diagnostics.
    • Existing methods may lack sensitivity or specificity for low-abundance heparin-binding proteins.

    Purpose of the Study:

    • To describe a novel method for the sensitive detection and quantification of picomole amounts of heparin-binding proteins.
    • To characterize the factors influencing heparin binding to proteins immobilized on nitrocellulose.

    Main Methods:

    • Proteins spotted on nitrocellulose membranes.
    • Incubation with radio-labeled 125I-heparin.
    • Detection by radioautography and quantification via scanning densitometry.
    • Protein quantification using amido black staining and densitometry.

    Main Results:

    • The method successfully detects and quantifies picomole levels of heparin-binding proteins.
    • Heparin binding is time-dependent.
    • Binding is modulated by metal ions, urea, and detergents.
    • Specific ions (Ca2+, Mg2+) and a zwitterionic detergent enhanced binding, while others (NaCl, urea, SDS, La3+) inhibited it.

    Conclusions:

    • The described assay provides a sensitive and versatile tool for identifying and characterizing heparin-binding proteins.
    • Understanding the influence of various chemical agents on heparin binding aids in assay optimization and interpretation.
    • This method is applicable to a wide range of heparin-binding proteins in biological samples.

    Related Experiment Videos