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[Fe]-Hydrogenase, Cofactor Biosynthesis and Engineering.

Francisco J Arriaza-Gallardo1, Yu-Cong Zheng1, Manuel Gehl1

  • 1Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Straße 10, 35043, Marburg, Germany.

Chembiochem : a European Journal of Chemical Biology
|September 6, 2023
PubMed
Summary
This summary is machine-generated.

[Fe]-hydrogenase and its iron-guanylylpyridinol (FeGP) cofactor are crucial for hydrogen metabolism. This review details FeGP biosynthesis via hcg genes and discusses enzyme engineering for future applications.

Keywords:
biosynthesiscofactorhydrogenasemetalloenzymesprotein structure

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Area of Science:

  • Biochemistry
  • Enzymology
  • Structural Biology

Background:

  • [Fe]-hydrogenase catalyzes critical H2 cleavage and hydride transfer.
  • The iron-guanylylpyridinol (FeGP) cofactor, featuring mononuclear Fe(II) with pyridinol and CO ligands, is essential for enzyme function.
  • FeGP biosynthesis is governed by the hmd co-occurring (hcg) gene cluster.

Purpose of the Study:

  • To review the properties of [Fe]-hydrogenase and its FeGP cofactor.
  • To summarize the biosynthesis pathways of the FeGP cofactor.
  • To discuss potential engineering strategies for [Fe]-hydrogenase and the FeGP cofactor.

Main Methods:

  • Structure-to-function analysis utilizing protein crystal structures.
  • Enzyme assays to determine catalytic properties.
  • In vitro biosynthesis assays for radical S-adenosyl methionine enzymes (HcgA, HcgG).

Main Results:

  • Detailed characterization of HcgB, HcgC, HcgD, HcgE, and HcgF functions through structural and enzymatic studies.
  • Reported catalytic properties of HcgA and HcgG, novel radical enzymes involved in FeGP biosynthesis.
  • Comprehensive review of [Fe]-hydrogenase and FeGP cofactor properties and biosynthesis.

Conclusions:

  • The hcg gene cluster encodes essential proteins for FeGP cofactor biosynthesis.
  • Understanding enzyme structures and functions facilitates the study of FeGP biosynthesis.
  • Future engineering of [Fe]-hydrogenase and FeGP cofactor holds promise for biotechnological applications.