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Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
Published on: March 21, 2018
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Comprehensive analysis of bisphenol analogues in complex water using a group-targeting aptamer engineered by base
Siqi Wu1, Siyao Liu1, Zhiming Wang1
1School of Chemical Science and Engineering, Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration, Ministry of Education, Tongji Hospital, Tongji University, Shanghai 200092, People's Republic of China.
Journal of Hazardous Materials
|September 6, 2023
Summary
Researchers developed a novel aptamer to detect multiple bisphenol analogues (BPs) in water. This group-targeting binder enables sensitive monitoring of these environmental hormones, improving detection in complex aquatic samples.
Area of Science:
- Environmental Science
- Analytical Chemistry
- Biotechnology
Background:
- Bisphenol analogues (BPs) are environmental hormones with endocrine-disrupting and reproductive toxicity.
- Accurate monitoring of BPs in aquatic environments is challenging due to co-existing contaminants.
- A specific, group-targeting binder is needed for comprehensive BP detection.
Purpose of the Study:
- To identify a group-targeting aptamer capable of binding multiple bisphenol analogues.
- To develop a sensitive aptasensor for the comprehensive analysis of BPs in aquatic samples.
Main Methods:
- Engineered a bisphenol A (BPA)-specific aptamer through base mutations.
- Utilized molecular docking to select a group-targeting aptamer for BPs (BPA, BPB, BPE, BPF).
- Constructed an electrochemical aptamer-based sensor (aptasensor) for BP detection.
Main Results:
- Identified a group-targeting BPs-aptamer with similar affinities for multiple BPs (binding constants ~2.0 × 10^6 to 2.7 × 10^6 / M).
- The aptasensor achieved a low detection limit of 6.7 pM for BPs.
- Demonstrated excellent anti-interference capabilities against up to 100-fold higher concentrations of co-existing contaminants.
Conclusions:
- The engineered group-targeting BPs-aptamer facilitates comprehensive analysis of multiple bisphenol analogues.
- This approach offers a sensitive and robust method for monitoring BPs in complex environmental matrices.
- The study provides valuable insights for identifying and monitoring a class of endocrine-disrupting pollutants.
