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Updated: Aug 10, 2026

Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses
Published on: February 25, 2011
[Selective modification of the free amino groups of the antibiotic ristomycin A]
Abstract:
Selective chemical modification of ristomycin A (I) by the free NH2 groups was performed. 6 different N-acyl and N-aminoacyl derivatives of I were obtained. 5 of them showed antibacterial activity within 0.4-10.0 micrograms/ml against 4 test microbes. The biological activity of the derivatives depended on the substituent type and the molecule total charge: mono-N-L-isoleucyl-I with the charge of +2 had the highest activity and N,N'-disuccinyl-I had the lowest activity.
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