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Phenotypic and functional distinctions between the TH2+ and JRA+ T cell subsets in man
Journal of Immunology (Baltimore, Md. : 1950)
|July 1, 1979
Summary
This study differentiates human T cell subsets, finding that TH2+ and JRA+ cells represent distinct populations. The TH2+ subset, not the JRA+ subset, mediates cell-mediated lympholysis, clarifying T cell subset functions.
Area of Science:
- Immunology
- Cell Biology
Background:
- Distinct human peripheral blood T cell subsets have been identified using various antisera.
- Understanding the precise relationships between these subsets is crucial for immunological research.
Purpose of the Study:
- To investigate the relationship between the T helper 2 (TH2+) and Juvenile Rheumatoid Arthritis (JRA+) T cell subsets in human peripheral blood.
- To determine if these subsets represent overlapping or distinct cell populations.
Main Methods:
- Purification of T cells using Sephadex G-200 anti-F(ab)2' affinity chromatography and E-rosetting.
- Fractionation of T cells into TH2+ and TH2- subsets using indirect immunofluorescence and Fluorescence-Activated Cell Sorting (FACS).
- Reciprocal fractionation into JRA+ and JRA- subsets followed by analysis with anti-TH2+ antisera.
Main Results:
- Approximately 40-45% of the TH2- subset was JRA+, while less than 5% of the TH2+ subset was JRA+.
- FACS analysis revealed no TH2+ cells within the JRA+ population.
- The JRA- population contained all TH2+ cells, indicating distinct populations.
Conclusions:
- The TH2+ and JRA+ T cell subsets represent distinct populations within human peripheral blood.
- The TH2+ subset, not the JRA+ subset, contains the effector cells responsible for cell-mediated lympholysis.