A lysosome-targeted triazole near-infrared cyanine fluorescent probe for in vivo long-term cell tracking

In vivo visualization of cell migration and engraftment in small animals provides crucial information for the development and clinical translation of cell-based therapies. Therefore, a good quality near-infrared (NIR) fluorescent probe with high optical properties and excellent cellular retention ability is desired for in vivo cell tracking. Herein, we designed and synthesized a lysosome-targeted triazole NIR cyanine fluorescent probe, named IR780-NT-NH₂, for in vivo long-term cell tracking. For the design, the heptamethine cyanine dye IR780 was used as the NIR fluorescent skeleton to ensure that the absorption and emission wavelengths fall within the NIR window. The substituent N-triazole group endowed the probe with high photostability and brightness. It has a quantum yield of 17.3% and the brightness remained above 85% after continuous illumination for 30 min. Due to the primary amine docking group, IR780-NT-NH₂ has excellent lysosomal targeting and retention abilities as it becomes protonated in an acidic environment. The strong signal strength of IR780-NT-NH₂ was maintained in well-shaped cells after an additional 12 h incubation. Moreover, this NIR probe exhibited ideal cellular permeability and biosafety. Finally, we realized long-term cell tracking with IR780-NT-NH₂ labeled PC-3 cells using a NIR imaging system. The present study provides evidence that IR780-NT-NH₂ exhibits ideal optical properties, excellent cellular permeation and retention, and good biosafety, which are useful for in vivo long-term observation of cells, and thus it shows promising potential for visualization in cell-based therapy.