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Updated: Jul 17, 2025
Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
Published on: January 17, 2015
Antibody Selection via Phage Display in Microtiter Plates
Stephan Steinke1, Kristian Daniel Ralph Roth1, Maximilian Ruschig1
1Institut für Biochemie, Biotechnologie und Bioinformatik, Departments Biotechnology and Medical Biotechnology, Technische Universität Braunschweig, Braunschweig, Germany.
Phage display is a key technology for generating monoclonal antibodies. This review details a rapid protocol for antibody selection and identification in under two weeks, including an on-rate panning method.
Area of Science:
- Biotechnology
- Immunology
- Molecular Biology
Background:
- Phage display is a leading in vitro method for producing monoclonal human antibodies.
- It is a crucial technology for recombinant antibody selection, with applications in research, diagnostics, and therapeutics.
Approach:
- This review presents a high-throughput, 96-well plate-based protocol for antibody selection.
- The described method facilitates rapid antibody "panning," screening, and identification within two weeks.
Key Points:
- The protocol enables efficient selection and identification of monoclonal antibodies.
- An on-rate panning strategy is included for selecting antibodies with fast binding kinetics.
Conclusions:
- This streamlined phage display protocol accelerates the discovery of high-quality monoclonal antibodies.
- The method supports the development of novel antibody-based research tools, diagnostics, and therapeutics.
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