Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Immune Response Against Viral Pathogens01:29

Immune Response Against Viral Pathogens

815
The immune system's response to viral infections is a complex and coordinated process involving natural killer (NK) cells, T cell-mediated responses, and antibody-mediated responses.
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
815

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Advanced green electrochemical pH sensor using phyto-cardamom modified electrode.

Food chemistry·2026
Same author

Replacing immunisation-dependent antibody reagent production: Challenges under the UK <i>Replacing</i> <i>Animals in</i> <i>Science</i> 2025 strategy.

Alternatives to laboratory animals : ATLA·2026
Same author

Next-Generation MXene-Hydrogel Hybrids: Frontiers in Biosensing and Environmental Surveillance.

ACS applied bio materials·2026
Same author

Designing <i>in situ</i> nanostructured MWCNT-phloroglucinol modified webs for electrochemical-based dual screening of stress biomarkers.

Physical chemistry chemical physics : PCCP·2026
Same author

Electrocatalytic advancements with trimetallic nanoparticles: design strategies and roadmap.

Nanoscale advances·2026
Same author

Quantifying vector diversion effects in zoonotic systems: A modelling framework for arbovirus transmission between reservoir and dead-end hosts.

PLoS computational biology·2025

Related Experiment Video

Updated: Jul 17, 2025

Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity
12:31

Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity

Published on: May 1, 2018

13.8K

Mapping Polyclonal Antibody Responses to Infection Using Next-Generation Phage Display.

Maria T Tsoumpeli1, Anitha Varghese1, Jonathan P Owen2

  • 1School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington, Leicestershire, UK.

Methods in Molecular Biology (Clifton, N.J.)
|September 7, 2023
PubMed
Summary
This summary is machine-generated.

Next-generation phage display (NGPD) identifies pathogen epitopes by mapping polyclonal antibody responses to infection. This method reveals diverse mimotopes, aiding serological assay and vaccine development.

Keywords:
AntibodyBioinformatic analysisBiopanningEpitope mappingNext generation sequencingPeptide ELISAPhage display

More Related Videos

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
12:55

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

Published on: January 17, 2015

18.6K
A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits
12:36

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits

Published on: February 16, 2014

34.1K

Related Experiment Videos

Last Updated: Jul 17, 2025

Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity
12:31

Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity

Published on: May 1, 2018

13.8K
Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
12:55

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

Published on: January 17, 2015

18.6K
A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits
12:36

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits

Published on: February 16, 2014

34.1K

Area of Science:

  • Immunology
  • Molecular Biology
  • Bioinformatics

Background:

  • Peptide phage display traditionally maps monoclonal antibodies.
  • Next-generation sequencing (NGS) enhances phage display for high-throughput screening.
  • Mapping polyclonal antibody responses is crucial for understanding infection immunity.

Purpose of the Study:

  • To detail methods for applying next-generation phage display (NGPD) to map polyclonal antibody responses.
  • To identify mimotopes representing pathogen epitopes recognized by infected individuals' antibodies.
  • To facilitate novel serological assay development and vaccine design.

Main Methods:

  • Utilizing next-generation phage display (NGPD) for mass screening of peptide-antibody binding events.
  • Applying NGPD to analyze antibody repertoires from individuals with specific infections.
  • Identifying and characterizing mimotopes that mimic pathogen epitopes.

Main Results:

  • Successful application of NGPD to map polyclonal antibody responses to infection.
  • Identification of diverse mimotopes, including linear, conformational, and post-translationally modified epitopes.
  • Demonstration of NGPD's capability to represent complex immunological recognition.

Conclusions:

  • NGPD is a powerful tool for mapping complex antibody responses to pathogens.
  • Identified mimotopes can inform the development of new diagnostic tools and vaccines.
  • This methodology advances our understanding of host-pathogen interactions and immune recognition.