Molecularly imprinted dispersive micro solid-phase extraction coupled with high-performance liquid chromatography for...

A new dummy template-based molecularly imprinted dispersive micro solid-phase extraction (MI-d-µSPE) coupled with HPLC-FLD developed for the simultaneous determination of four aflatoxins (B₁, B₂, G₁, G₂) in various food matrices. The synthesized MIP was used as a dispersive solid-phase extraction (dSPE) sorbent for aflatoxins extraction. The chemometric approach was used to identify the optimum conditions of dSPE. The results showed the amount of MIP sorbent (55 mg), adsorption time (12.5 min), and %ACN (75%) were significant extraction parameters. The method has a detection limit in the range of 0.059-0.208 µg kg^-1 and a quantification limit in the range of 0.197-0.694 µg kg^-1 for aflatoxins. The intra- and inter-day precision was less than 5%, and recoveries were 79.1-109.4%. The expanded uncertainty of the developed method was found to be 2.9-22.8%. The new MI-d-µSPE with HPLC-FLD method was applied for 37 food matrices.