Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Intracellular Movement of Viruses and Bacteria01:10

Intracellular Movement of Viruses and Bacteria

2.9K
Intracellular bacteria and viruses often comprise a group of highly infectious pathogens that can cause several diseases. Bacterial pathogens include those belonging to the genus Rickettsia responsible for conditions such as rocky mountain spotted fever and the Mediterranean spotted fever; Chlamydia, a genus responsible for a sexually transmitted disease; Coxiella burnetii, an agent responsible for Q fever. Viral pathogens include vaccinia—a poxvirus, and herpes simplex virus—a...
2.9K
Microtubule Formation01:23

Microtubule Formation

5.8K
Microtubules are dynamic structures that undergo continuous assembly and disassembly. They originate from specialized multi-protein complexes known as microtubule organizing centers or MTOCs. Within the MTOC, the point of origin of the microtubule is known as the minus end, while the end radiating outward is the plus end. Microtubules serve two primary functions — the organization of spindle complexes to separate sister chromatids during mitotic or meiotic cell division and the formation...
5.8K
Studying the Cytoskeleton01:17

Studying the Cytoskeleton

6.3K
The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...
6.3K
Assembly of Cytoskeletal Filaments01:18

Assembly of Cytoskeletal Filaments

21.0K
Cytoskeletal filaments are polymeric forms of smaller protein subunits. However, individual cytoskeletal filaments may easily disassemble or associate with other similar filaments to form rigid structures. Microfilaments, made of actin monomers, rely on actin-binding proteins to form bundles and create networks of individual actin filaments. Microtubules rely on microtubule-associated proteins (MAPs) to form sturdy cylindrical structures. However, the proteins involved in forming complex...
21.0K
Cytoskeletal Coordination in Cell Migration01:32

Cytoskeletal Coordination in Cell Migration

4.8K
A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
4.8K
Introduction to the Cytoskeleton01:33

Introduction to the Cytoskeleton

26.8K
Overview of the Cytoskeleton
The cytoskeleton is a network of protein filaments present within the cell, having three distinct filaments ̶   microfilaments, microtubules, and intermediate filaments. Each has characteristic features that distinguish them, including the dynamics of their assembly and disassembly, mechanical properties, polarity, and the type of molecular motors associated with them. Earlier, they were thought to be present only in eukaryotic cells; however, their...
26.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

TARDBP as a regulator of HIV-1 assembly and infection: a review of targeting the viral capsid precursor Pr55Gag and limiting viral core entry.

Cell communication and signaling : CCS·2026
Same author

Cohesin reconstitution and homologous recombination repair of DNA double-strand breaks in late mitosis.

eLife·2025
Same author

The ZIKV NS5 Protein Aberrantly Alters the Tubulin Cytoskeleton, Induces the Accumulation of Autophagic p62 and Affects IFN Production: HDAC6 Has Emerged as an Anti-NS5/ZIKV Factor.

Cells·2024
Same author

TDP-43 Controls HIV-1 Viral Production and Virus Infectiveness.

International journal of molecular sciences·2023
Same author

Nanomaterials to combat SARS-CoV-2: Strategies to prevent, diagnose and treat COVID-19.

Frontiers in bioengineering and biotechnology·2022
Same author

Contribution of the HIV-1 Envelope Glycoprotein to AIDS Pathogenesis and Clinical Progression.

Biomedicines·2022

Related Experiment Video

Updated: Jul 16, 2025

Imaging of HIV-1 Envelope-induced Virological Synapse and Signaling on Synthetic Lipid Bilayers
11:45

Imaging of HIV-1 Envelope-induced Virological Synapse and Signaling on Synthetic Lipid Bilayers

Published on: March 8, 2012

12.3K

HIV Infection: Shaping the Complex, Dynamic, and Interconnected Network of the Cytoskeleton.

Romina Cabrera-Rodríguez1, Silvia Pérez-Yanes1, Iria Lorenzo-Sánchez1

  • 1Laboratorio de Inmunología Celular y Viral, Unidad de Farmacología, Sección de Medicina, Facultad de Ciencias de la Salud, Universidad de La Laguna (ULL), 38200 La Laguna, Spain.

International Journal of Molecular Sciences
|September 9, 2023
PubMed
Summary

Human immunodeficiency virus type 1 (HIV-1) manipulates host cell

Keywords:
HIV-1 Env complexHIV-1 Pr55GagHIV-1 infectioncontrol of the infectioncytoskeleton adaptors and motor proteinscytoskeleton dynamicsviremia and progression

More Related Videos

Single-Cell Characterization of Calcium Influx and HIV-1 Infection using a Multiparameter Optofluidic Platform
07:15

Single-Cell Characterization of Calcium Influx and HIV-1 Infection using a Multiparameter Optofluidic Platform

Published on: May 18, 2021

3.2K
Determination of Molecular Structures of HIV Envelope Glycoproteins using Cryo-Electron Tomography and Automated Sub-tomogram Averaging
07:29

Determination of Molecular Structures of HIV Envelope Glycoproteins using Cryo-Electron Tomography and Automated Sub-tomogram Averaging

Published on: December 1, 2011

41.6K

Related Experiment Videos

Last Updated: Jul 16, 2025

Imaging of HIV-1 Envelope-induced Virological Synapse and Signaling on Synthetic Lipid Bilayers
11:45

Imaging of HIV-1 Envelope-induced Virological Synapse and Signaling on Synthetic Lipid Bilayers

Published on: March 8, 2012

12.3K
Single-Cell Characterization of Calcium Influx and HIV-1 Infection using a Multiparameter Optofluidic Platform
07:15

Single-Cell Characterization of Calcium Influx and HIV-1 Infection using a Multiparameter Optofluidic Platform

Published on: May 18, 2021

3.2K
Determination of Molecular Structures of HIV Envelope Glycoproteins using Cryo-Electron Tomography and Automated Sub-tomogram Averaging
07:29

Determination of Molecular Structures of HIV Envelope Glycoproteins using Cryo-Electron Tomography and Automated Sub-tomogram Averaging

Published on: December 1, 2011

41.6K

Area of Science:

  • Cell biology
  • Virology
  • Molecular biology

Background:

  • The human immunodeficiency virus type 1 (HIV-1) lifecycle involves complex interactions with the host cell cytoskeleton.
  • Cytoskeletal elements like actin filaments (AFs), intermediate filaments (IFs), and microtubules (MTs) are crucial for cellular processes exploited by HIV-1.
  • HIV-1 utilizes host cell cytoskeletal machinery for viral entry, intracellular transport, assembly, and release.

Purpose of the Study:

  • To review how HIV-1 regulates the host cell cytoskeleton to facilitate viral infection.
  • To analyze the mechanisms by which HIV-1 manipulates cytoskeletal organization and dynamics.
  • To discuss the implications of these interactions for HIV-1 pathogenesis and therapeutic strategies.

Main Methods:

  • This is a review article, synthesizing existing research on HIV-1 and the cytoskeleton.
  • Analysis of scientific literature focusing on molecular interactions between HIV-1 components and host cytoskeletal proteins.
  • Examination of how HIV-1 envelope (Env) and accessory proteins influence cytoskeletal dynamics.

Main Results:

  • HIV-1 Env binding to CD4/CCR5/CXCR4 receptors initiates cytoskeletal changes for fusion and entry.
  • Intracellular transport of the viral core to the nucleus relies on cytoskeletal tracks, adaptors, and motors.
  • HIV-1 regulates cytoskeleton dynamics for efficient viral Pr55Gag expression, virion assembly, and release.

Conclusions:

  • HIV-1 actively remodels the host cell cytoskeleton to create a permissive environment for infection.
  • Understanding these cytoskeletal manipulations is key to comprehending HIV-1 pathogenesis.
  • Targeting HIV-1-induced cytoskeletal alterations presents a potential avenue for novel therapeutic interventions.