Related Experiment Video
Updated: Jul 16, 2025
07:32
Standardized Histomorphometric Evaluation of Osteoarthritis in a Surgical Mouse Model
Published on: May 6, 2020
12.2K
Relations between Structure/Composition and Mechanics in Osteoarthritic Regenerated Articular Tissue: A Machine
Matteo Berni1, Francesca Veronesi2, Milena Fini3
1Medical Technology Laboratory, IRCCS Istituto Ortopedico Rizzoli, Via Di Barbiano 1/10, 40136 Bologna, Italy.
International Journal of Molecular Sciences
|September 9, 2023
Summary
Mesenchymal stem cell treatments for osteoarthritis (OA) show potential in improving cartilage viscoelasticity. Stromal vascular fraction (SVF) was the only treatment to limit OA
Area of Science:
- Orthopedics
- Regenerative Medicine
- Biomaterials Science
Background:
- Osteoarthritis (OA) is a degenerative joint disease characterized by cartilage breakdown and loss of viscoelastic properties.
- Orthobiologics, including mesenchymal stem cells (MSCs), are being investigated for OA treatment.
- Understanding the relationship between tissue structure, composition, and cartilage function is crucial for developing effective OA therapies.
Purpose of the Study:
- To investigate the relationship between articular tissue structure/composition and cartilage viscoelasticity in a large animal model of early osteoarthritis (OA).
- To evaluate the efficacy of different mesenchymal stem cell preparations (adipose-derived mesenchymal stem cells (ADSCs), stromal vascular fraction (SVF), and amniotic endothelial cells (AECs)) in treating OA.
- To identify key biomarkers and structural parameters influencing cartilage viscoelasticity in OA.
Main Methods:
- A large animal model of induced knee OA in 24 sheep was used.
- Sheep were treated with ADSCs, SVF, or AECs and euthanized at 3 or 6 months post-treatment.
- Analyses included synovial fluid biochemistry, cartilage histology/immunohistochemistry/histomorphometry, and cartilage viscoelasticity measurements.
- Machine learning models (VSURF and XGB) were employed for variable selection and interpretation.
Main Results:
- A correlation was found between cartilage elastic modulus and cartilage thickness (CT), synovial fluid interleukin 6 (IL6), and prostaglandin E2 (PGE2).
- Cartilage relaxation time was associated with CT and PGE2.
- Stromal vascular fraction (SVF) treatment demonstrated a protective effect against the detrimental impact of OA on cartilage viscoelastic properties.
Conclusions:
- This study highlights potential relationships between specific biochemical markers (IL6, PGE2), structural parameters (CT), and cartilage viscoelasticity in OA.
- SVF emerged as a promising orthobiologic treatment, mitigating OA-induced viscoelastic deterioration.
- Findings provide a basis for future research into advanced regenerative targets for OA treatment.
Related Concept Videos
01:20Structural Classification of Joints
3.5K
Joints, also known as articulations, are classified based on their structural characteristics, i.e., based on whether the articulating surfaces of the adjacent bones are directly connected by fibrous connective tissue or cartilage, or whether the articulating surfaces contact each other within a fluid-filled joint cavity. These differences serve to divide the joints of the body into three structural classifications.
A fibrous joint is where the adjacent bones are united by fibrous connective...
A fibrous joint is where the adjacent bones are united by fibrous connective...
3.5K
01:09Functional Classification of Joints
4.2K
Functional Classification of Joints
The functional classification of joints is determined by the amount of mobility between the adjacent bones. Joints are functionally classified as a synarthrosis or immobile joint, an amphiarthrosis or slightly moveable joint, or as a diarthrosis, a freely moveable joint. Fibrous and cartilaginous joints can be functionally classified as either synarthroses or amphiarthroses, whereas all synovial joints are classified as diarthroses.
Synarthrosis
An...
The functional classification of joints is determined by the amount of mobility between the adjacent bones. Joints are functionally classified as a synarthrosis or immobile joint, an amphiarthrosis or slightly moveable joint, or as a diarthrosis, a freely moveable joint. Fibrous and cartilaginous joints can be functionally classified as either synarthroses or amphiarthroses, whereas all synovial joints are classified as diarthroses.
Synarthrosis
An...
4.2K
01:18Classification of Bones
5.7K
The bones of the human skeletal system are of varied shapes, sizes, and functions. They can be classified based on their shape and function into four major classes: long bones, short bones, flat bones, and irregular bones. Some classifications include a fifth type, the sesamoid bones, as a separate class, whereas others categorize them under short bones.
Long and Short Bones
The appendicular skeleton, particularly the upper and lower limbs, is primarily made of long and short bones. The...
Long and Short Bones
The appendicular skeleton, particularly the upper and lower limbs, is primarily made of long and short bones. The...
5.7K