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Related Experiment Video

Updated: Jul 16, 2025

Cellular Redox Profiling Using High-content Microscopy

Cellular Redox Profiling Using High-content Microscopy

Published on: May 14, 2017

ChromatoShiny: an interactive R/Shiny App for plotting chromatography profiles.

Natalia Y Kochanova¹, Maria Alba Abad¹, Petra Vizjak²

¹Wellcome Centre for Cell Biology, The University of Edinburgh, Edinburgh, Scotland, EH9 3BF, UK.

Wellcome Open Research

|September 11, 2023

Summary

This summary is machine-generated.

This study introduces an interactive R Shiny app for analyzing liquid chromatography data. The tool enhances chromatogram interpretation and publication figure generation, requiring no programming experience.

Keywords:

Chromatography Shiny apps Äkta machines

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Area of Science:

Biochemistry
Biophysics
Structural Biology
Chromatography

Background:

Unicorn™ software on Äkta liquid chromatography instruments generates chromatogram profiles for purified biological macromolecules.
Existing software lacks advanced annotation, customization, and export functionalities for detailed analysis.

Purpose of the Study:

To develop an interactive application for improved interpretation of chromatograms.
To facilitate the generation of publication-quality figures from chromatography data.

Main Methods:

Utilized the R Shiny framework to build an interactive web application.
The app integrates baseline fitting, fraction highlighting, and plot zooming functionalities.

Main Results:

The application enables users to fit baselines and highlight specific fractions or elution volumes.
Users can easily zoom into plots for detailed examination of chromatography profiles.
An interactive app is freely available at https://ChromatoShiny.bio.ed.ac.uk.

Conclusions:

The developed app requires no programming expertise, making it accessible to a wide range of users.
It empowers chromatography users to generate informative and annotated chromatogram plots efficiently.
The tool simplifies the process of data visualization for publication and analysis.