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Related Experiment Video

Updated: Jul 16, 2025

Evaluation of Biomaterials for Bladder Augmentation using Cystometric Analyses in Various Rodent Models
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Engineering pedicled vascularized bladder tissue for functional bladder defect repair.

Mingming Yu1,2,3, Jiasheng Chen1,2,3, Lin Wang1,2,3

  • 1Department of Urology Shanghai Children's Hospital, Shanghai Jiao Tong University Shanghai China.

Bioengineering & Translational Medicine
|September 11, 2023
PubMed
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This summary is machine-generated.

This study demonstrates that integrating buccal mucosa grafts with vascularized smooth muscle tissue creates a functional, full-layer bladder construct. This engineered bladder shows promise for effective bladder reconstruction and functional recovery.

Area of Science:

  • Regenerative Medicine
  • Biomaterials Science
  • Urology

Background:

  • Engineered bladder constructs aim to replicate native bladder structure and function for substitution therapies.
  • Previous work established pedicled vascularized smooth muscle tissue for bladder reconstruction.

Purpose of the Study:

  • To assess the integration of buccal mucosa graft (BMG) with vascularized smooth muscle tissue.
  • To develop a full-layer, pedicled, vascularized bladder construct for bladder reconstruction.

Main Methods:

  • Fabrication of a pedicled vascularized bladder construct using BMG and vascularized smooth muscle tissue.
  • Reconstruction of full-thickness bladder defects using different constructs (pedicled capsule, nonpedicled, pedicled).
  • Histological (Picrosirius Red staining, immunohistochemistry) and functional assessments (compliance, ductility).
Keywords:
bladderbuccal mucosacapsulecell sheetstissue engineeringvascularization

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Main Results:

  • BMG integrated successfully, forming a full-layer construct with good survival, vascularization, and barrier function.
  • The pedicled construct group exhibited minimal fibrosis, high smooth muscle proportion, and dense vascularization.
  • Only the pedicled construct group showed a continuous mucosal layer, improved bladder compliance, and ductility.

Conclusions:

  • Buccal mucosa graft integration with vascularized smooth muscle tissue yields a promising pedicled, vascularized bladder construct.
  • This approach facilitates structural remodeling and functional recovery, offering an alternative for bladder reconstruction.