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M13 Bacteriophage-Assisted Recognition and Signal Spatiotemporal Separation Enabling Ultrasensitive Light Scattering
Hao Fang1, Yaofeng Zhou1, Yanbing Ma1
1State Key Laboratory of Food Science and Resources, School of Food Science and Technology, Nanchang University, Nanchang 330047, P. R. China.
ACS Nano
|September 12, 2023
Summary
This study introduces a novel M13 phage-assisted immunoassay for ultrasensitive, rapid quantification of trace analytes. The method enhances detection sensitivity by over 10,000 times compared to traditional assays.
Area of Science:
- Biotechnology
- Analytical Chemistry
- Nanotechnology
Background:
- Traditional immunoassays face limitations in sensitivity due to the trade-off between molecular recognition and signal amplification.
- The size effect of nanoprobes in traditional methods restricts the simultaneous optimization of sensitivity and specificity.
- There is a growing need for ultrasensitive and rapid quantitative analysis of trace target analytes.
Purpose of the Study:
- To develop a versatile immunoassay system for ultrasensitive quantitative detection of low-abundance target analytes.
- To overcome the sensitivity limitations of traditional immunoassay-based detection methods.
- To enable rapid quantification of analytes in real samples with enhanced sensitivity.
Main Methods:
- Utilized M13 phage-assisted immunorecognition and signal transduction with spatiotemporal separation.
- Combined M13 phage-assisted light scattering signal fluctuations of gold nanoparticles (AuNPs) with gold in situ growth (GISG) technology.
- Implemented a biotin-streptavidin amplification scheme for enhanced sensitivity.
Main Results:
- Achieved ultrasensitive and rapid quantification of ochratoxin A and alpha-fetoprotein at the subfemtomolar level within 50 minutes.
- Demonstrated a sensitivity enhancement of approximately 4 orders of magnitude compared to traditional phage-based ELISA.
- Detected severe acute respiratory syndrome coronavirus 2 spike protein down to the attomolar range using biotin-streptavidin amplification.
Conclusions:
- The developed M13 phage-assisted immunoassay offers a significant advancement in ultrasensitive quantitative detection.
- The synergistic combination of M13 phage-mediated leverage effect and GISG-amplified light scattering provides a powerful platform for trace analyte analysis.
- This strategy presents a promising direction for developing next-generation ultrasensitive diagnostic tools.
