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In vitro function of radiocolloid-labelled monocytes
European Journal of Nuclear Medicine
|January 1, 1986
Summary
This study found that indium-111-iron-colloid labeling of human monocytes does not affect their in vitro function. However, rapid radioactivity loss limits their use in long-term in vivo monocyte kinetic studies.
Area of Science:
- Nuclear Medicine
- Hematology
- Immunology
Background:
- Monocyte kinetic studies are crucial for understanding hematological malignancies.
- Accurate labeling of monocytes is essential for reliable kinetic studies.
Purpose of the Study:
- To evaluate the in vitro function of human monocytes labeled with 111In-Fe-colloid.
- To assess the suitability of this labeling method for monocyte kinetic studies in hematological malignancies.
Main Methods:
- Human monocytes were labeled with 111In-Fe-colloid.
- In vitro function tests included phagocytosis of opsonized zymosan particles.
- Monocyte differentiation into macrophages was assessed in glass tissue chambers.
Main Results:
- Labeled and non-labeled monocytes showed no significant differences in in vitro function.
- Cell viability remained above 90% after labeling.
- A rapid loss of cell-bound radioactivity was observed during in vitro culture.
Conclusions:
- The 111In-Fe-colloid labeling procedure does not impair in vitro monocyte function.
- The rapid in vitro loss of radioactivity makes this method unsuitable for long-term in vivo monocyte kinetic studies.