Scalable mesenchymal stem cell enrichment from bone marrow aspirate using deterministic lateral displacement (DLD)...

Area of Science:

• Regenerative Medicine
• Biotechnology
• Cell Therapy

Background:

• Mesenchymal stem cells (MSCs) are crucial for regenerative medicine.
• Conventional bone marrow aspirate (BMA) processing for MSCs is inefficient, time-consuming, and yields low cell counts.
• Current methods suffer from osmotic stress and high centrifugation forces, impacting cell viability.

Purpose of the Study:

• To develop a scalable microfluidic technology for efficient MSC isolation from BMA.
• To overcome the limitations of traditional centrifugation methods for MSC harvesting.
• To enable faster and more effective cell therapy preparation.

Main Methods:

• Development of a microfluidic device utilizing deterministic lateral displacement (DLD) for cell sorting.
• Passive, label-free separation based on morphological differences between MSCs and other blood components.
• Implementation of a multi-chip DLD system for high-throughput processing of BMA.

Main Results:

• The DLD platform processed 2.5 mL of BMA in approximately 20 minutes.
• Achieved a 2-fold increase in MSC recovery compared to conventional centrifugation.
• Demonstrated effective separation of MSCs from platelets and red blood cells (RBCs).

Conclusions:

• The developed DLD microfluidic technology provides a rapid and efficient method for MSC isolation from BMA.
• This platform has the potential to significantly improve MSC yield and streamline cell therapy workflows.
• Enables faster and more efficient isolation of MSCs for clinical applications.