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Related Concept Videos

Amperometry: Overview01:10

Amperometry: Overview

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Amperometry is a technique commonly used to measure the concentration of specific analytes in a solution by monitoring the electric current generated during an electrochemical reaction. It involves applying a constant potential between a working electrode and a reference electrode to measure the resulting current, which is proportional to the concentration of the analyte. The Clark oxygen electrode operates based on this principle of amperometry. It consists of a cathode and an anode enclosed...
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Simultaneous detection of dopamine and ascorbic acid by using a thread-based microfluidic device and multiple pulse

Vanessa W Dos Santos1, Gustavo Martins1, Jeferson L Gogola1

  • 1Laboratory of Electrochemical Sensors (LabSensE), Chemistry Department, Federal University of Paraná (UFPR), CEP 81.531-980, Curitiba, PR, Brazil. luiz1berto@ufpr.br.

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This study introduces a novel 3D-printed microfluidic device for simultaneously detecting ascorbic acid (AA) and dopamine (DA). The affordable μTED device uses textile threads and multiple pulse amperometry for accurate quantification in samples.

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Area of Science:

  • Electrochemistry
  • Analytical Chemistry
  • Microfluidics

Background:

  • Microfluidic devices coupled with electrochemical detectors offer portability and efficiency.
  • Simultaneous detection of ascorbic acid (AA) and dopamine (DA) is crucial in various analytical applications.
  • Existing methods may require complex separation or modified electrodes.

Purpose of the Study:

  • To develop an affordable and user-friendly 3D-printed microfluidic device (μTED) for simultaneous AA and DA detection.
  • To utilize textile threads as microfluidic channels and an unmodified screen-printed electrode (SPE) as a detector.
  • To implement multiple pulse amperometry (MPA) for accurate quantification without prior separation.

Main Methods:

  • Fabrication of a 3D-printed microfluidic device (μTED) using textile threads.
  • Amperometric detection using an unmodified screen-printed electrode (SPE).
  • Application of multiple pulse amperometry (MPA) with specific potentials (+0.65 V and -0.10 V) and a correction factor for simultaneous analysis.

Main Results:

  • Achieved linear ranges of 50–900 μmol L⁻¹ for both AA and DA.
  • Obtained sensitivities of 2.24 μA L mmol⁻¹ for AA and 5.09 μA L mmol⁻¹ for DA.
  • Reported limits of detection (LOD) of 2.60 μmol L⁻¹ for AA and 1.54 μmol L⁻¹ for DA.
  • Demonstrated successful analysis in spiked blood serum samples with a 70% recovery rate.

Conclusions:

  • The developed μTED device provides a simple, cost-effective, and efficient platform for simultaneous AA and DA determination.
  • MPA effectively enables simultaneous quantification without chemical modification or separation.
  • The method shows promise for practical applications in biological sample analysis.