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Related Experiment Video

Updated: Jul 16, 2025

High Throughput, Real-time, Dual-readout Testing of Intracellular Antimicrobial Activity and Eukaryotic Cell Cytotoxicity

High Throughput, Real-time, Dual-readout Testing of Intracellular Antimicrobial Activity and Eukaryotic Cell Cytotoxicity

Published on: November 16, 2016

High-throughput functional trait testing for bacterial pathogens.

Zachary R Stromberg¹, Shelby M B Phillips¹, Kristin M Omberg¹

¹Chemical and Biological Signatures Group, Pacific Northwest National Laboratory , Richland, Washington, USA.

|September 13, 2023

Summary

This summary is machine-generated.

High-throughput functional assays offer a powerful approach to identify bacterial pathogen virulence traits, complementing whole-genome sequencing for comprehensive characterization and novel microbe detection.

Keywords:

bacterial pathogen high-throughput methods microbial functional traits pathogen agnostic detection pathogenesis

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Last Updated: Jul 16, 2025

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Area of Science:

Microbiology
Pathogen Discovery
Genomics

Background:

Functional traits impact microorganism fitness and metabolic function.
Characterizing bacterial pathogens using functional virulence traits is of increasing interest.
Traditional single-trait phenotyping is time-consuming; whole-genome sequencing (WGS) has limitations in predicting all functional traits.

Purpose of the Study:

To advocate for high-throughput functional assays as a prominent method for bacterial pathogen characterization.
To highlight the importance of functional trait identification for pathogen-agnostic approaches.
To discuss advancements in high-throughput methods for understanding pathogens.

Main Methods:

Review and discussion of six key areas of functional trait testing.
Exploration of high-throughput assay advancements.
Comparison with traditional phenotyping and WGS-based prediction.

Main Results:

High-throughput functional assays can overcome limitations of traditional methods and WGS alone.
These methods enable population-scale characterization of bacterial pathogens.
Advancing these assays is crucial for detecting novel microbes and understanding virulence.

Conclusions:

High-throughput functional trait identification should be prioritized for bacterial pathogen characterization.
This approach is essential for developing pathogen-agnostic detection strategies.
Further advancements in high-throughput methods will deepen our understanding of microbial pathogens.