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Updated: Oct 2, 2025

Imaging InlC Secretion to Investigate Cellular Infection by the Bacterial Pathogen Listeria monocytogenes

Imaging InlC Secretion to Investigate Cellular Infection by the Bacterial Pathogen Listeria monocytogenes

Published on: September 19, 2013

Engineered Cell Line Imaging Assay Differentiates Pathogenic from Non-Pathogenic Bacteria.

Shelby M B Phillips¹, Carson Bergstrom¹, Brian Walker¹

¹Chemical and Biological Signatures Group, Pacific Northwest National Laboratory, Richland, WA 99352, USA.

Pathogens (Basel, Switzerland)

|February 26, 2022

Summary

This summary is machine-generated.

This study engineered lung cells with fluorescent reporters to track signaling pathways. Researchers found that pathogenic bacteria rapidly disrupt extracellular signal-related kinase (ERK) and FOS-related antigen 1 (Fra1) signaling, enabling sensitive detection.

Keywords:

bio-agent agnostic signatures detection fluorescent reporters mammalian cell engineering pathogenicity protein kinase

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Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells

Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells

Published on: August 5, 2016

Area of Science:

Bacterial pathogenesis
Cell signaling
Host-pathogen interactions

Background:

Cell culture models are crucial for understanding bacterial manipulation of host signaling pathways.
Genetic engineering enables fluorescent reporters for signaling pathways, but their application in pathogen biology is limited.

Purpose of the Study:

To genetically engineer a lung cell line with fluorescent reporters for extracellular signal-related kinase (ERK) and FOS-related antigen 1 (Fra1).
To evaluate host cell signaling responses to pathogenic and non-pathogenic bacteria.
To establish a rapid and sensitive screening method for bacterial pathogens based on ERK-Fra1 signaling.

Main Methods:

Engineered a lung cell line with fluorescent reporters for ERK and Fra1 signaling.
Inoculated engineered and wild-type cells with various bacterial species (Acinetobacter baylyi, Klebsiella pneumoniae, Pseudomonas aeruginosa, Streptococcus agalactiae, Staphylococcus epidermidis).
Utilized multi-mode imaging and the alamarBlue cell viability assay to assess cell death and signaling pathway disruption.

Main Results:

Pathogenic Pseudomonas aeruginosa induced significant cell death compared to non-pathogenic Staphylococcus epidermidis.
Fra1 signaling disruption occurred within 4 hours of inoculation with bacterial pathogens, while non-pathogenic bacteria caused delayed disruption.
ERK-Fra1 signaling changes effectively differentiated between pathogenic and non-pathogenic bacterial infections at low levels.

Conclusions:

Genetically engineered cell lines with fluorescent reporters provide a sensitive tool for studying host-pathogen interactions.
ERK-Fra1 signaling serves as a rapid biomarker for detecting pathogenic bacterial infections.
This approach facilitates the development of novel screening methods for bacterial pathogens.