Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Fixation and Sectioning01:03

Fixation and Sectioning

4.4K
Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
4.4K
Differential Staining Technique01:26

Differential Staining Technique

70
Differential staining is an essential microbiological technique that exploits variations in cell wall structures to classify and identify microorganisms. It facilitates the distinction of bacteria, aiding in diagnostic and research applications. Two of the most widely used differential staining methods are Gram staining and acid-fast staining, both of which rely on the chemical and structural differences in bacterial cell walls.Gram Staining TechniqueGram staining differentiates bacteria by...
70
Simple Staining Technique01:24

Simple Staining Technique

127
OverviewStaining techniques in microscopy enhance the visualization of microorganisms by increasing contrast and allowing the differentiation of cellular structures. Simple staining is one of the fundamental methods used to observe the basic morphological characteristics of microorganisms, including their size, shape, and arrangement. This method relies on the application of a single dye to stain the entire cell, producing a clear contrast between the cell and the background.FixationFixation is...
127
Special Staining Techniques01:13

Special Staining Techniques

48
Specialized staining techniques play a vital role in microbiology by enabling the visualization of specific bacterial structures that remain undetectable with standard microscopy methods. These techniques not only enhance the structural visualization of bacterial cells but also provide critical insights into their pathogenicity and classification. Additionally, they support diagnostic and research endeavors in microbiology by identifying key bacterial features.Capsule Staining for Virulence...
48

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Serum Selenium Concentration in Neurosurgery Patients Receiving Parenteral Nutrition.

Journal of Nippon Medical School = Nippon Ika Daigaku zasshi·2026
Same author

Pancreas-contactless open gastrectomy for gastric cancer and postoperative pancreatic complications: A retrospective cohort study.

Surgical oncology·2026
Same author

Cerebral Infarction Diagnosed as Isolated Cardiac Sarcoidosis.

NMC case report journal·2026
Same author

Appetite-preserving gastrectomy attenuates early postoperative deterioration of bone quality after surgery in esophagogastric junction cancer.

Surgical oncology·2026
Same author

Frailty and Severe White Matter Lesions Are Risk Factors for Surgical Treatment for Unruptured Cerebral Aneurysm in Older Adults.

Neurologia medico-chirurgica·2026
Same author

Molecular and clinical features of a Japanese medulloblastoma cohort: Subgroup-specific prognostic stratification using economical/accessible diagnostic methods.

Brain pathology (Zurich, Switzerland)·2026

Related Experiment Video

Updated: Jul 16, 2025

Optimization of Laser-Capture Microdissection for the Isolation of Enteric Ganglia from Fresh-Frozen Human Tissue
08:28

Optimization of Laser-Capture Microdissection for the Isolation of Enteric Ganglia from Fresh-Frozen Human Tissue

Published on: June 14, 2018

8.7K

Experience Using Gentian Violet-Free Dyes for Tissue Visualization.

Fumihiro Matano1, Yasuo Murai1, Yohei Nounaka1

  • 1Department of Neurological Surgery, Nippon Medical School, Tokyo, Japan.

Journal of Neurological Surgery. Part A, Central European Neurosurgery
|September 13, 2023
PubMed
Summary

C.I. Basic Violet 4 (BV4) offers a safe alternative to gentian violet ink for surgical marking. This gentian violet-free dye demonstrated similar color visibility without causing microvascular thrombosis in vascular anastomosis.

More Related Videos

Histological-Based Stainings Using Free-Floating Tissue Sections
06:45

Histological-Based Stainings Using Free-Floating Tissue Sections

Published on: August 25, 2020

18.8K
Combining Lipophilic dye, in situ Hybridization, Immunohistochemistry, and Histology
13:05

Combining Lipophilic dye, in situ Hybridization, Immunohistochemistry, and Histology

Published on: March 17, 2011

26.2K

Related Experiment Videos

Last Updated: Jul 16, 2025

Optimization of Laser-Capture Microdissection for the Isolation of Enteric Ganglia from Fresh-Frozen Human Tissue
08:28

Optimization of Laser-Capture Microdissection for the Isolation of Enteric Ganglia from Fresh-Frozen Human Tissue

Published on: June 14, 2018

8.7K
Histological-Based Stainings Using Free-Floating Tissue Sections
06:45

Histological-Based Stainings Using Free-Floating Tissue Sections

Published on: August 25, 2020

18.8K
Combining Lipophilic dye, in situ Hybridization, Immunohistochemistry, and Histology
13:05

Combining Lipophilic dye, in situ Hybridization, Immunohistochemistry, and Histology

Published on: March 17, 2011

26.2K

Area of Science:

  • Vascular Surgery
  • Surgical Oncology
  • Biomaterials Science

Background:

  • Gentian violet ink is a common surgical skin marker, but poses risks like carcinogenicity and venous injury leading to microvascular thrombosis.
  • Current surgical practices necessitate safer alternatives for skin marking and vessel visualization.

Purpose of the Study:

  • To compare the efficacy and safety of C.I. Basic Violet 4 (BV4), a gentian violet-free dye, against traditional gentian violet ink.
  • To evaluate BV4's utility in terms of color visibility and its impact on microvascular thrombosis formation in vascular anastomosis.

Main Methods:

  • A comparative study involving 20 cases with 3 vascular anastomoses.
  • BV4 and gentian violet ink were used to delineate bone cutting lines, superior temporal artery, and middle cerebral artery.

Main Results:

  • BV4 exhibited comparable color visibility to gentian violet ink.
  • No instances of thrombus formation were observed at the vascular anastomosis sites when utilizing BV4.

Conclusions:

  • C.I. Basic Violet 4 (BV4) is a viable alternative to gentian violet ink for surgical applications.
  • BV4 demonstrated no adverse effects, specifically the absence of microvascular thrombus formation, in vascular anastomosis procedures.