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Updated: Aug 15, 2026

On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System
14:15

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Published on: February 23, 2018

Monoclonal antibodies specific for Corynebacterium sepedonicum, the causative agent of potato ring rot

W E Magee, C F Beck, S S Ristow

    Hybridoma
    |January 1, 1986
    PubMed

    BALB/c mice were immunized with Corynebacterium sepedonicum, and spleen cells from the immunized animals were fused with cells of the mouse myeloma line P3-X63-Ag8.653. Several hybridoma cell cultures were selected for further study. Monoclonal CS-B-5 was specific for C. sepedonicum and did not react significantly with other closely related phytopathogenic corynebacteria in an enzyme-linked immunosorbent assay (ELISA). As few as 10(3) organisms could be detected. This approach should prove useful for developing improved diagnostic procedures for a number of bacterial plant pathogens.

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