Comprehensive analysis of OsJAZ gene family deciphers rhizobacteria-mediated nutrient stress modulation in rice

The JASMONATE-ZIM DOMAIN (JAZ) repressors are crucial proteins in jasmonic acid signaling pathway that are critical for plant growth. Therefore, the present study aimed to identify and characterize OsJAZs in the rice genome, revealing their structural attributes, regulatory elements, miRNA interactions, and subcellular localization. 23 JAZ transcripts across the 6 chromosomes of rice genome were identified having conserved domains and different physiochemical characteristics. Phylogenetically classified into five clades, they showed highest syntenic relationship with P. virgatum. The non-synonymous/synonymous values ranged from 0.44 to 1.21 suggesting purifying/stabilizing selection in OsJAZs. The study examined the 1.5 kb promoter region for cis-regulatory elements, and also identified 92 miRNAs targets. Furthermore, homology modeling provided insights into the 3D-structures of JAZ proteins while in-silico gene expression analysis revealed their functional diversity in various tissues and developmental stages. Additionally, qRT-PCR analysis highlighted their involvement in stress adaptation to sub-optimum nutrient conditions induced by plant-beneficial rhizobacteria Bacillus amyloliquefaciens (SN13) in two rice varieties. Distinct OsJAZ expression patterns in the two varieties correlated with altered root architecture, xylem structure, and lignification. These findings affirmed that specific up-or down-regulation of OsJAZs might play critical role in SN13 induced changes in the two varieties that enabled them to survive under stress.