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Published on: August 7, 2019
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Comparison of fine-needle aspiration techniques
Jason P Dufour1, Carolina Allers1, Faith Schiro1
1Tulane National Primate Research Center, Covington, Louisiana, USA.
Journal of Medical Primatology
|September 15, 2023
Summary
Technique B, a novel fine-needle aspiration (FNA) method, yielded the highest cell count and strong viability in rhesus macaque studies. This approach offers a superior alternative for cell recovery in research settings.
Area of Science:
- Biomedical research
- Primate studies
- Cellular analysis
Background:
- Fine-needle aspiration (FNA) has evolved since 1912, offering an alternative to excisional biopsy.
- Despite limitations, FNA remains a valuable technique for various assays.
Purpose of the Study:
- To compare four distinct FNA techniques in rhesus macaques.
- To evaluate cell recovery and viability across different FNA methods.
Main Methods:
- Four FNA techniques were assessed in rhesus macaques.
- Standard 6 mL syringe and 1.5-inch 22-gauge needle were utilized.
- Total live cells and cell viability were key evaluation metrics.
Main Results:
- Technique B demonstrated the highest total cell count.
- Technique B achieved the second-highest cell viability.
- This technique involved removing the needle for forced air expulsion and flushing.
Conclusions:
- Technique B is identified as the superior method for FNA in this study.
- The findings suggest improved cell recovery and viability with Technique B.
