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Expression and Related Mechanism of miR-744 in Ischemia-reperfusion Rat Model
1Department of Cardiovascular Medicine, the Affiliated Hospital of Inner Mongolia Medical University, Hohhot, China. jing_guoqiang@163.com.
Abstract:
To study the expression of miR-744 in the rat model of ischemia-reperfusion (I-R) and its related mechanism. Seventy-five Wistar rats were randomly divided into 3 groups: sham operation group (SOG), model group (MG) and miR-744 group, with 25 in each group. The expression levels of IL-1β, IL-6 and TNF-α were observed by Western Blot after the model preparation, while miR-744 expression was detected by reverse transcription polymerase chain reaction (RT-PCR). The cerebral infarction volume of rats in the MG was significantly larger than that in the miR-744 group (P<0.05). The MG exhibited a markedly higher brain tissue water content than the SOG and the miR-744 group (P<0.05). When compared within the latter two groups, the brain tissue water content in the SOG was significantly lower than that in the miR-744 group (P<0.05). As to miR-744 expression, the relative expression of miR-744 in the brain tissue of the MG was the lowest among the three groups. When compared within the remaining two groups, the miR-744 expression of the miR-744 group was remarkably higher than that of the SOG (P<0.05). In terms of the expression levels of inflammatory factors, the expressions of IL-1β, IL-6 and TNF-α in the brain tissue of the SOG and the miR-744 group were significantly lower than those in the MG, and those of the SOG were significantly lower than that of the miR-744 group. MiR-744 may be involved in the development and progression of I-R in rats, and its mechanism may be related to the regulation of inflammatory response.
Insights
This study investigated microRNA-744 (miR-744) in a rat model of ischemia-reperfusion (I-R) injury. Findings suggest miR-744 plays a role in I-R by regulating inflammatory responses, potentially offering therapeutic insights.
Area of Science:
- Neuroscience
- Molecular Biology
- Biochemistry
Background:
- Ischemia-reperfusion (I-R) injury is a significant cause of brain damage.
- The role of microRNAs (miRNAs) in I-R pathophysiology is an emerging area of research.
- Understanding specific miRNA involvement, like miR-744, is crucial for developing targeted therapies.
Purpose of the Study:
- To investigate the expression of miR-744 in a rat model of cerebral I-R injury.
- To elucidate the potential mechanism of miR-744 in regulating inflammatory responses during I-R.
- To assess the impact of miR-744 modulation on brain damage markers.
Main Methods:
- Establishment of a rat model of cerebral I-R.
- Random group allocation: Sham Operation Group (SOG), Model Group (MG), and miR-744 Group.
- Quantitative analysis of miR-744 expression using reverse transcription polymerase chain reaction (RT-PCR).
- Assessment of inflammatory markers (IL-1β, IL-6, TNF-α) via Western Blot.
- Measurement of cerebral infarction volume and brain tissue water content.
Main Results:
- The MG showed significantly larger cerebral infarction volume and higher brain tissue water content compared to the miR-744 group.
- miR-744 expression was lowest in the MG and significantly higher in the miR-744 group compared to the SOG.
- Expressions of IL-1β, IL-6, and TNF-α were significantly elevated in the MG compared to both SOG and miR-744 groups.
Conclusions:
- miR-744 expression is altered in rat cerebral I-R injury, with decreased levels observed in the model group.
- Modulation of miR-744 appears to reduce cerebral infarction volume and brain edema.
- The findings suggest miR-744 is involved in the development and progression of I-R injury, likely through the regulation of inflammatory responses.
