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Identification of type D Pasteurella multocida by counterimmunoelectrophoresis
Abstract:
A counterimmunoelectrophoresis (CIE) test was applied to serotype 35 isolates of type D Pasteurella multocida recovered from 32 cases of atrophic rhinitis (in swine) and 3 cases of snuffles (in rabbits). The CIE test was compared with the indirect hemagglutination (IHA) and acriflavine (AF) tests. Results of the CIE test correlated 100% with those of the IHA test whereas results of the AF test correlated 91.43% with those of the IHA test. The CIE test was rapid and simpler to perform compared with the IHA test and more sensitive than the AF test. Cross-reactions were not encountered with capsular antigens of P. multocida types A, B, and E in the CIE test. The CIE test was not found to be suitable for typing type A P. multocida strains.
Insights
Counterimmunoelectrophoresis (CIE) is a rapid and sensitive method for identifying type D Pasteurella multocida in swine and rabbits. This test shows excellent correlation with the indirect hemagglutination (IHA) test.
Area of Science:
- Veterinary Microbiology
- Immunological Assays
- Bacterial Pathogenesis
Background:
- Pasteurella multocida is a significant pathogen causing diseases like atrophic rhinitis in swine and snuffles in rabbits.
- Accurate and rapid identification of Pasteurella multocida serotypes is crucial for effective disease management and control.
- Existing diagnostic methods may have limitations in terms of speed, simplicity, or sensitivity.
Purpose of the Study:
- To evaluate the efficacy of counterimmunoelectrophoresis (CIE) for serotyping type D Pasteurella multocida isolates.
- To compare the performance of CIE with established methods like indirect hemagglutination (IHA) and acriflavine (AF) tests.
- To assess the sensitivity, specificity, and practicality of the CIE test for routine diagnostics.
Main Methods:
- Counterimmunoelectrophoresis (CIE) was employed to serotype 35 isolates of type D Pasteurella multocida.
- Isolates were obtained from swine with atrophic rhinitis and rabbits with snuffles.
- The CIE test results were compared against those obtained from indirect hemagglutination (IHA) and acriflavine (AF) tests.
Main Results:
- The CIE test demonstrated 100% correlation with the IHA test for serotyping.
- The AF test showed a 91.43% correlation with the IHA test.
- CIE was found to be faster and simpler than IHA, and more sensitive than AF.
- No cross-reactions were observed with capsular antigens of P. multocida types A, B, and E using CIE.
- CIE was not suitable for typing type A P. multocida strains.
Conclusions:
- Counterimmunoelectrophoresis (CIE) is a highly effective, rapid, and sensitive diagnostic tool for identifying type D Pasteurella multocida.
- CIE offers a reliable alternative to IHA and a more sensitive option than AF for this specific serotype.
- While CIE is valuable for type D, its limitations for type A necessitate continued use of other methods for comprehensive Pasteurella multocida typing.