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This lesson illustrates the role of deuterium substitution in simplifying the NMR spectrum of compounds comprising labile protons. One method employed is the use of deuterium. Amongst the three isotopes of hydrogen, deuterium (2H) has a nucleus composed of one proton and one neutron. When the D2O solvent is added to a pure dry ethanol solution, its labile proton is substituted with deuterium.
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Updated: Jul 16, 2025

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Deep Eutectic Solvent-Based Highly Sensitive Turn-On Fluorescent Probe for D2O.

Sushil M Patil1,2, Santosh Kumar Gupta1,2, Dibakar Goswami1,2

  • 1Fuel Chemistry Division, Radiochemistry Division, Bio Organic Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400085, India.

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|September 18, 2023
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Summary
This summary is machine-generated.

Researchers developed a novel, sensitive fluorescent sensor for detecting ultratrace levels of heavy water (D2O). This cost-effective method also determines isotopic purity, crucial for various industries.

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Area of Science:

  • Analytical Chemistry
  • Materials Science

Background:

  • Heavy water (D2O) is vital in spectroscopy, nuclear energy, and biological applications.
  • A need exists for robust, affordable, and sensitive D2O detection methods.

Purpose of the Study:

  • To develop a highly sensitive, turn-on fluorescent sensor for ultratrace D2O detection.
  • To utilize a laboratory-prepared deep eutectic solvent (DES) for D2O sensing.
  • To enable simultaneous sensing and isotopic purity determination of D2O.

Main Methods:

  • A novel deep eutectic solvent (DES) was synthesized.
  • The sensor leverages the unique fluorescence emission of the DES.
  • Detection is based on the rapid exchange of labile DES protons with deuterium from D2O.
  • Fluorescence measurements were compared with Fourier-transform infrared (FT-IR) spectroscopy.

Main Results:

  • A highly sensitive turn-on fluorescent sensor for D2O was successfully developed.
  • The sensor achieved a limit of detection of 0.079% (v/v) or 870 ppm for D2O.
  • Isotopic purity measurements using the DES sensor closely matched conventional FT-IR results.

Conclusions:

  • The developed DES-based fluorescent sensor offers a sensitive and cost-effective method for D2O analysis.
  • This sensor enables both quantitative detection and isotopic purity assessment of heavy water.
  • It represents a significant advancement for heavy water monitoring strategies.