Polymeric nanoparticle-based electrochemical sensor for the detection of CA 125

Area of Science:

• Biomaterials Science
• Electrochemistry
• Biosensor Technology

Background:

• CA 125 is a crucial biomarker for ovarian cancer detection and monitoring.
• Existing methods for CA 125 determination can be time-consuming and lack sensitivity.
• Development of novel nanomaterials and electrochemical techniques is essential for improved diagnostics.

Purpose of the Study:

• To develop novel Cys-graft-p(HEMA) nanomaterials for CA 125 determination.
• To establish a new electrochemical method for sensitive and rapid CA 125 detection.
• To evaluate the performance of the fabricated biosensor for clinical applications.

Main Methods:

• Cys-graft-p(HEMA) nanomaterials synthesized via emulsion polymerization and grafting.
• Electrode surface modification and optimization for electrochemical analysis.
• Electrochemical detection of CA 125 using the fabricated sensor system.

Main Results:

• Cys-graft-p(HEMA) nanomaterials exhibit 50 nm spherical morphology with 0.011 mmol L-cysteine per gram.
• Linear determination range of 5-400 U/mL with high correlation (R=0.9935).
• Achieved a low detection limit (LOD) of 1.87 U/mL and quantification limit (LOQ) of 5.62 U/mL.
• The sensor demonstrated excellent repeatability, accuracy, reality, and storage stability.
• Rapid determination time of 10 minutes.

Conclusions:

• Cys-graft-p(HEMA) nanomaterials represent a novel and effective material for CA 125 biosensing.
• The developed electrochemical method offers a rapid, sensitive, and accurate approach for CA 125 determination.
• This biosensor system holds significant potential for early disease diagnosis and patient treatment monitoring.