Selective nanosensor based on folic acid imprinted nanostructures

Area of Science:

• Electrochemistry
• Nanotechnology
• Biochemistry

Background:

• Folic acid is crucial for metabolism, nucleotide synthesis, and methylation, with deficiencies leading to various disorders.
• Accurate folic acid determination methods are essential for diagnosing and managing related health conditions.
• Current methods for folic acid analysis require innovative, cost-effective, and sensitive approaches.

Purpose of the Study:

• To develop a novel folic acid imprinted nanostructure-modified surface-printed carbon electrode (SPCE) for folic acid determination.
• To create a highly selective and sensitive nanosensor for detecting folic acid in human blood serum.
• To establish a rapid and cost-effective electrochemical method for folic acid analysis.

Main Methods:

• Synthesis and characterization of folic acid imprinted nanostructures (FA-Imp-poly(MPTS-rGO-co-NAT)) using FTIR, SEM-EDS, and AFM.
• Fabrication of a nanosensor by modifying SPCE with the synthesized nanostructures.
• Electrochemical analysis using differential pulse voltammetry (DPV) and cyclic voltammetry (CV).

Main Results:

• The developed FA-imprinted nanosensor exhibited a low detection limit (LOD) of 7.54 ng/mL and a quantification limit (LOQ) of 25.14 ng/mL.
• High precision was demonstrated with relative standard deviation (RSD) values as low as 0.092% in DPV measurements.
• The nanosensor showed selectivity and sensitivity for folic acid determination in synthetic serum samples.

Conclusions:

• A novel, simple, selective, and rapid folic acid imprinted nanosensor has been successfully developed.
• The developed electrochemical nanosensor is suitable for the quantitative determination of folic acid in biological samples like human blood serum.
• This innovative approach offers a promising tool for clinical diagnostics and metabolic studies related to folic acid.