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Dynamic Multiplex Tissue Imaging in Inflammation Research.

Stefan Uderhardt1,2,3, Georgiana Neag1,2,3, Ronald N Germain4,5

  • 1Department of Medicine 3-Rheumatology and Immunology, Friedrich-Alexander-University Erlangen-Nürnberg and Universitätsklinikum Erlangen, Erlangen, Germany.

Annual Review of Pathology
|September 18, 2023
PubMed
Summary

Two-photon microscopy (2PM) enables in vivo imaging of deep tissues, revealing dynamic immune cell behaviors crucial for understanding inflammation. This technology helps correlate cellular dynamics with in situ function and molecular patterns within complex tissue environments.

Keywords:
dynamic imagingintravital imagingmultiplex imagingtissue imagingtwo-photon microscopy

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Area of Science:

  • Immunology
  • Cell Biology
  • Biomedical Imaging

Background:

  • Inflammation involves dynamic immune cell interactions within complex tissue environments.
  • Assessing these cellular dynamics in vivo is crucial due to limitations of ex vivo models.
  • Deep tissue imaging is necessary to observe these processes far below organ surfaces.

Purpose of the Study:

  • To illustrate how two-photon microscopy (2PM) facilitates in vivo studies of immune cell dynamics.
  • To connect observed immune cell behaviors to their in situ functions and governing molecular patterns.
  • To review practical aspects and potential confounding factors of 2PM in biological research.

Main Methods:

  • Utilizing two-photon excitation lasers integrated with existing imaging systems.
  • Performing long-term, deep-tissue imaging in vivo.
  • Analyzing studies that apply 2PM to investigate immune cell behavior in inflammatory contexts.

Main Results:

  • 2PM allows visualization of immune cell migration and interactions deep within tissues.
  • Studies demonstrate the link between immune cell dynamics and their functional roles in situ.
  • Molecular patterns influencing immune cell behavior in vivo have been elucidated using 2PM.

Conclusions:

  • Two-photon microscopy is a powerful tool for studying dynamic immune cell behavior in vivo.
  • Understanding in situ cell dynamics is essential for comprehending inflammatory processes.
  • Careful consideration of 2PM methodologies is needed to ensure reliable scientific conclusions.