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Rose long noncoding RNA lncWD83 promotes flowering by modulating ubiquitination of the floral repressor RcMYC2L
Chen Yeqing1,2,3, Lu Jun1,2,3, Wang Weinan1,2,3
1Key Laboratory of Landscaping, Ministry of Agriculture and Rural Affairs, Nanjing 210095, China.
Plant Physiology
|September 18, 2023
Summary
A long noncoding RNA, lncWD83, from rose plants promotes flowering by controlling the ubiquitination and degradation of a floral repressor protein, RcMYC2L. This reveals a novel regulatory pathway involving lncRNAs and E3 ubiquitin ligases in plant development.
Area of Science:
- Plant Molecular Biology
- Epigenetics
- Plant Physiology
Background:
- Long noncoding RNAs (lncRNAs) are increasingly recognized for their regulatory roles in plant signaling pathways.
- The interaction between lncRNAs and E3 ubiquitin ligases in regulating plant flowering remains largely unexplored.
- Understanding these interactions is crucial for deciphering complex developmental processes in plants.
Purpose of the Study:
- To investigate the role of the lncRNA lncWD83 in regulating flowering time in rose (Rosa chinensis).
- To elucidate the molecular mechanism by which lncWD83 influences flowering, focusing on its interaction with E3 ubiquitin ligases and floral repressors.
- To uncover a novel regulatory pathway involving lncRNAs in ubiquitin-mediated proteolysis controlling plant flowering.
Main Methods:
- Virus-induced gene silencing (VIGS) was used to assess the function of lncWD83 and RcPUB11 in rose plants.
- In vitro pull-down assays were employed to determine the association between lncWD83 and the E3 ubiquitin ligase RcPUB11.
- Co-immunoprecipitation and in vitro ubiquitination assays were performed to study the interaction and ubiquitination of RcMYC2L.
- Quantitative real-time PCR (qRT-PCR) and promoter assays were conducted to analyze the regulation of RcFT transcription.
Main Results:
- Silencing lncWD83 significantly delayed flowering in rose plants.
- lncWD83 was found to associate with the E3 ubiquitin ligase RcPUB11.
- Knockdown of RcPUB11 phenocopied the late-flowering phenotype of lncWD83-silenced plants, and RcMYC2L was ubiquitinated by RcPUB11.
- lncWD83 enhanced the RcPUB11-mediated degradation of the floral repressor RcMYC2L, which normally represses RcFT transcription, thereby promoting flowering.
Conclusions:
- The lncRNA lncWD83 promotes flowering in rose by facilitating the ubiquitination and proteasomal degradation of the floral repressor RcMYC2L.
- This study reveals a novel mechanism where lncRNAs modulate E3 ubiquitin ligase activity to control plant flowering time.
- The findings provide new insights into the crosstalk between lncRNAs and the ubiquitin-proteasome system in regulating plant development.
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