Related Experiment Video
Updated: Jul 16, 2025
07:18
Highly-Multiplexed Tissue Imaging with Raman Dyes
Published on: April 21, 2022
2.9K
Translating genomic tools to Raman spectroscopy analysis enables high-dimensional tissue characterization on
Manuel Sigle1, Anne-Katrin Rohlfing1, Martin Kenny2,3
1Department of Cardiology and Angiology, University Hospital Tuebingen, Eberhard Karls University Tuebingen, 72076, Tuebingen, Germany.
Nature Communications
|September 19, 2023
Summary
Raman microspectroscopy reveals molecular details in tissues, complementing spatial transcriptomics. This label-free method analyzes cell and matrix composition, aiding disease research.
Area of Science:
- Biomedical Engineering
- Molecular Biology
- Biochemistry
Background:
- Spatial transcriptomics offers genetic insights into tissue reorganization.
- Biomolecular composition analysis lags behind genomic studies, creating an information gap.
- Raman microspectroscopy provides high-resolution, untargeted spatiomolecular information.
Purpose of the Study:
- To demonstrate spatially resolved Raman "spectromics" for molecular-level tissue analysis.
- To repurpose bioinformatic tools from transcriptomics for "spectromics" data.
- To reveal homogeneity, heterogeneity, and dynamics of cell-matrix interactions.
Main Methods:
- Spatially resolved Raman microspectroscopy was applied to histological sections.
- Bioinformatic analysis tools, typically used for transcriptomics, were adapted.
- Murine myocardial infarction and cardiac hypertrophy models were studied.
Main Results:
- Identification of myocardial subclusters approaching pathological areas.
- Definition of the surrounding metabolic and cellular (immune) landscape.
- Demonstration of Raman "spectromics" for label-free, non-invasive tissue characterization.
Conclusions:
- Spatially resolved Raman "spectromics" bridges the gap in biomolecular sample analysis.
- The approach enables profound characterization of histological samples.
- This method allows for combination with downstream analyses on the same specimen.
More Related Videos
Related Concept Videos
01:20Raman Spectroscopy: Overview
443
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
443
01:26Raman Spectroscopy Instrumentation: Overview
450
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
450
02:24Ribosome Profiling
3.6K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.6K