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Updated: Jul 16, 2025

Author Spotlight: A Simple and Cost-Effective Method for Leukocyte Analysis in Semen
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Lipid peroxidation in avian semen.

A Partyka1, A Babapour2, M Mikita1

  • 1Wroclaw University of Environmental and Life Sciences, Faculty of Veterinary Medicine, Department of Reproduction and Clinic of Farm Animals, pl. Grunwaldzki 49, 50-366 Wroclaw, Poland.

Polish Journal of Veterinary Sciences
|September 20, 2023
PubMed
Summary

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Oxidative stress causes lipid peroxidation (LPO) in avian sperm, damaging fertilization capacity. This review covers LPO effects and common methods for evaluating this damage in bird semen.

Area of Science:

  • Reproductive Biology
  • Oxidative Stress Research
  • Avian Science

Background:

  • Oxidative stress is a primary cause of sperm chromatin damage, leading to infertility and embryo development failure in avian species.
  • Lipid peroxidation (LPO), the oxidation of polyunsaturated fatty acids (PUFAs), damages cell membranes and is particularly detrimental to avian sperm due to high PUFA content.
  • This susceptibility to peroxidative damage significantly impacts sperm fertilization capacity in birds.

Approach:

  • This review introduces the concept of lipid peroxidation (LPO) in avian semen.
  • It details the detrimental effects of LPO on avian sperm quality and function.
  • The review summarizes established methods for evaluating LPO and associated damage in both fresh and stored avian semen.

Key Points:

Keywords:
avian semenlipid peroxidationoxidative stresssemen cryopreservation

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  • Avian sperm are highly vulnerable to oxidative stress-induced lipid peroxidation (LPO) due to their membrane composition.
  • LPO negatively affects sperm function and fertilization potential in birds.
  • Commonly used evaluation methods include the TBARS assay for malondialdehyde (MDA) and fluorescence probes like CC 11-BODIPY 581/591 for direct LPO assessment.

Conclusions:

  • Understanding LPO in avian semen is crucial for addressing infertility issues.
  • Accurate evaluation of LPO is essential for assessing sperm quality in fresh and stored avian semen.
  • This review provides a foundation for researchers and practitioners in avian reproductive science.