Simultaneous electrophysiology and optogenetic perturbation of the same neurons in chronically implanted animals
Nathaniel R Kinsky1, Mihály Vöröslakos2, Jose Roberto Lopez Ruiz3
1Department of Anesthesiology and Neuroscience Graduate Program, University of Michigan Medical School, Ann Arbor, MI 48109, USA.
Abstract:
Micro-light-emitting-diode (μLED) silicon probes feature independently controllable miniature light-emitting-diodes (LEDs) embedded at several positions in each shank of a multi-shank probe, enabling temporally and spatially precise optogenetic neural circuit interrogation. Here, we present a protocol for performing causal and reproducible neural circuit manipulations in chronically implanted, freely moving animals. We describe steps for introducing optogenetic constructs, preparing and implanting a μLED probe, performing simultaneous in vivo electrophysiology with focal optogenetic perturbation, and recovering a probe following termination of an experiment. For complete details on the use and execution of this protocol, please refer to Watkins de Jong et al. (2023).1.
