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Related Experiment Videos

Platelet storage by temperature cycling.

M McGill

    Progress in Clinical and Biological Research
    |January 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

    Temperature cycling preserves platelet viability and function during storage. This method maintains microtubule reassembly capabilities, crucial for normal aggregation and enhanced recovery from hypotonic shock, unlike continuous cold storage.

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    Area of Science:

    • Hematology
    • Biotechnology
    • Cell Biology

    Background:

    • Platelet storage conditions significantly impact their in vitro viability, function, and ultrastructure.
    • Understanding optimal storage methods is critical for maintaining platelet efficacy for transfusion and research.

    Purpose of the Study:

    • To evaluate the effects of different storage temperatures (4°C, 22°C, and temperature cycling) on platelet viability and function.
    • To assess the ultrastructural changes, specifically microtubule reassembly capability, in stored platelets.

    Main Methods:

    • Platelets were stored under continuous 4°C, 22°C, and temperature-cycled conditions.
    • In vitro viability was assessed using hypotonic shock recovery.
    • Platelet function was evaluated by aggregation response.

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  • Ultrastructural integrity, including microtubule reassembly, was examined.

    • Main Results:

    • Continuous 4°C storage for 24 hours caused irreversible loss of discoid shape and microtubule reassembly capability.
    • Abnormal platelet aggregation and decreased viability correlated with the loss of microtubule reassembly.
    • Temperature-cycled storage maintained microtubule reassembly, leading to normal aggregation and enhanced viability.
    • Temperature-cycled platelets stored for 96 hours showed viability comparable to 24-hour storage at 4°C or 72-hour storage at 22°C.

    Conclusions:

    • Temperature cycling is a superior method for preserving platelet viability and function compared to continuous cold storage.
    • Maintaining microtubule reassembly capability is essential for preserving platelet function during storage.
    • Temperature cycling offers a promising strategy for extending the storage lifespan of functional platelets.