Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Association between dietary total antioxidant capacity and ulcerative colitis severity: a cross-sectional study.

Scientific reports·2026

Same author

Efficacy of microencapsulated organic acids-cinnamon blend as alternatives to antibiotic growth promoters: Assessing performance, gut histomorphometry, ileal digestibility, immunity, and antioxidant status in broilers.

Poultry science·2026

Same author

Protocol to stain phagocytic macrophages and assess phagocytosis function in phagocytic cells.

STAR protocols·2026

Same author

Exploring the association between hedonic hunger and obesogenic eating behaviors in females with overweight/obesity using machine learning methods.

Scientific reports·2026

Same author

Efficacy of Supplementary Guanidinoacetic Acid at Different Dietary Crude Protein Levels on the Productive Performance, Egg Quality, Intestinal Morphology, Antioxidative Status, and Serum Metabolites of Aged Laying Hens Exposed to High Ambient Temperatures.

Veterinary medicine and science·2026

Same author

<i>Myxococcus xanthus</i> for active matter studies: a tutorial for its growth and potential applications.

Soft matter·2025

Search research articles

Related Experiment Video

Updated: Jul 16, 2025

Analysis of Liver Microenvironment During Early Progression of Non-Alcoholic Fatty Liver Disease-Associated Hepatocellular Carcinoma in Zebrafish

Analysis of Liver Microenvironment During Early Progression of Non-Alcoholic Fatty Liver Disease-Associated Hepatocellular Carcinoma in Zebrafish

Published on: April 1, 2021

Microfluidic design for in-vitro liver zonation-a numerical analysis using COMSOL Multiphysics.

Reza Mahdavi¹, Sameereh Hashemi-Najafabadi², Mohammad Adel Ghiass³

¹Biotechnology Department, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, P.O. Box 14115-114, Iran.

Medical & Biological Engineering & Computing

|September 21, 2023

Summary

This study introduces a novel microfluidic chip design to replicate liver zonation in vitro. The optimized chip ensures proper oxygen and glucose distribution for accurate disease modeling and drug toxicity research.

Keywords:

COMSOL Liver zonation Microfluidics Numerical simulation Organ-on-a-chip

More Related Videos

Author Spotlight: Developing a Unique Modular Microphysiological System to Mimic Human Barrier Tissue

Author Spotlight: Developing a Unique Modular Microphysiological System to Mimic Human Barrier Tissue

Published on: February 16, 2024

Development and Characterization of In Vitro Microvessel Network and Quantitative Measurements of Endothelial [Ca2+]i and Nitric Oxide Production

Development and Characterization of In Vitro Microvessel Network and Quantitative Measurements of Endothelial [Ca2+]i and Nitric Oxide Production

Published on: May 19, 2016

Related Experiment Videos

Last Updated: Jul 16, 2025

Analysis of Liver Microenvironment During Early Progression of Non-Alcoholic Fatty Liver Disease-Associated Hepatocellular Carcinoma in Zebrafish

Analysis of Liver Microenvironment During Early Progression of Non-Alcoholic Fatty Liver Disease-Associated Hepatocellular Carcinoma in Zebrafish

Published on: April 1, 2021

Author Spotlight: Developing a Unique Modular Microphysiological System to Mimic Human Barrier Tissue

Author Spotlight: Developing a Unique Modular Microphysiological System to Mimic Human Barrier Tissue

Published on: February 16, 2024

Development and Characterization of In Vitro Microvessel Network and Quantitative Measurements of Endothelial [Ca2+]i and Nitric Oxide Production

Development and Characterization of In Vitro Microvessel Network and Quantitative Measurements of Endothelial [Ca2+]i and Nitric Oxide Production

Published on: May 19, 2016

Area of Science:

Biomedical Engineering
Cell Biology
Organ-on-a-Chip Technology

Background:

Current in vitro models lack accuracy for liver disease and toxicity studies.
Liver zonation, a key physiological feature, is challenging to replicate in vitro.
Organ-on-a-chip technology offers controlled environments for cell culture.

Purpose of the Study:

To propose and validate a microfluidic device for creating oxygen gradients to mimic liver zonation.
To identify optimal parameters for cell density and flow rate for physiological relevance.
To provide a tool for advanced in vitro liver studies and other gas-gradient-dependent cell research.

Main Methods:

Development of a microfluidic chip with an integrated gas channel for oxygen gradient generation.
Numerical simulation using COMSOL Multiphysics, incorporating cell-specific oxygen and glucose consumption rates.
Analysis of various flow rates and cell densities to determine optimal distribution.

Main Results:

Identification of optimal cell density and flow rate for uniform oxygen and glucose distribution.
Demonstration of achieving physiologically relevant oxygen and glucose concentrations within 24 hours and 30 minutes, respectively.
Validation of the microfluidic design's capability to establish controlled oxygen gradients.

Conclusions:

The proposed microfluidic design effectively recreates liver zonation in vitro.
This technology serves as a valuable tool for liver disease modeling, drug toxicity testing, and hypoxia studies.
The adaptable design is applicable to various cell studies requiring controlled gas concentration gradients.