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Published on: May 14, 2012
Which are the best murine models to study Eosinophilic Chronic Rhinosinusitis? A contemporary review
Francisco Leite-Santos1, Edwin Tamashiro1, Adriana de Andrade Batista Murashima1
1Universidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, Departamento de Oftalmologia, Otorrinolaringologia e Cirurgia de Cabeça e Pescoço, Divisão de Otorrinolaringologia, Ribeirão Preto, SP, Brazil.
Objective:
Mechanisms that lead to Eosinophilic Chronic Rhinosinusitis (ECRS) are not fully established in the literature. It is desirable to assess ECRS in a model that embraces most of the related events. This article reviewed the murine models for ECRS and compared them regarding eosinophilic polypoid formation.
Methods:
The authors reviewed the articles that included the terms "chronic rhinosinusitis" OR "chronic sinusitis" AND "animal model". We analyzed articles in English that evaluated both the number of polyps and the number of eosinophils in the sinus mucosa of mouse models.
Results:
We identified a total of 15 articles describing different models of ECRS that used BALB/c or C57BL/6 mice, and different triggers/stimulants such as Staphylococcus aureus Enterotoxin B (SEB) + Ovalbumin (OVA); House Dust Mite (HDM) ± Ovalbumin (OVA); and Aspergillus oryzae Protease (AP) + Ovalbumin (OVA). OVA associated with SEB was the commonest protocol to induce ECRS in both BALB/c and C57BL/6 mice, and it produced a robust response of eosinophilic nasal polyps in both. AP + OVA protocol also led to a good ECRS response. The other models were not considered adequate to produce eosinophilic polyps in mice.
Conclusion:
In conclusion, OVA associated with SEB seems to produce the most robust eosinophilic sinonasal inflammation.
Insights
Murine models for Eosinophilic Chronic Rhinosinusitis (ECRS) were reviewed. Ovalbumin (OVA) combined with Staphylococcus aureus Enterotoxin B (SEB) effectively induced robust eosinophilic polypoid formation in mice.
Area of Science:
- Immunology
- Otorhinolaryngology
- Animal Models
Background:
- Mechanisms of Eosinophilic Chronic Rhinosinusitis (ECRS) require further elucidation.
- Developing a comprehensive animal model is crucial for studying ECRS pathogenesis.
- Existing literature lacks a clear comparison of ECRS murine models.
Purpose of the Study:
- To review and compare existing murine models for Eosinophilic Chronic Rhinosinusitis (ECRS).
- To evaluate models based on their ability to induce eosinophilic polypoid formation.
- To identify the most effective model for ECRS research.
Main Methods:
- A systematic literature review was conducted using keywords 'chronic rhinosinusitis' OR 'chronic sinusitis' AND 'animal model'.
- Articles in English were analyzed for their evaluation of polyp count and eosinophil levels in sinus mucosa of mouse models.
- Fifteen articles describing various ECRS induction protocols in BALB/c or C57BL/6 mice were identified.
Main Results:
- Models utilizing Ovalbumin (OVA) with Staphylococcus aureus Enterotoxin B (SEB), House Dust Mite (HDM) ± OVA, and Aspergillus oryzae Protease (AP) + OVA were described.
- The OVA + SEB protocol was the most common and produced robust eosinophilic nasal polyps in both BALB/c and C57BL/6 mice.
- The AP + OVA protocol also showed a good ECRS response, while other models were less effective for inducing eosinophilic polyps.
Conclusions:
- Ovalbumin (OVA) associated with Staphylococcus aureus Enterotoxin B (SEB) appears to be the most effective protocol for inducing robust eosinophilic sinonasal inflammation in murine models.
- This model holds promise for further investigation into ECRS mechanisms.
- The findings guide the selection of appropriate animal models for ECRS research.
