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Overview Of Cell Separation And Isolation

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

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Updated: Jul 16, 2025

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Published on: July 10, 2016

Microfluidic pumps for cell sorting.

Leyla Akh¹, Diane Jung¹, William Frantz¹

¹Biomedical Engineering Program, University of Colorado, Boulder, Colorado 80309, USA.

Biomicrofluidics

|September 22, 2023

Summary

This summary is machine-generated.

Microfluidic cell sorting offers advantages over traditional methods, with six pumping techniques reviewed for their suitability in biological research and medical diagnostics. Future trends focus on integrated, high-throughput systems.

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Last Updated: Jul 16, 2025

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Area of Science:

Biotechnology
Medical Diagnostics
Bioengineering

Background:

Microfluidic cell sorting presents a miniaturized alternative to conventional cell sorters.
Key performance metrics include throughput, user-friendliness, and on-chip integration.
Applications span biological research and clinical diagnostics.

Purpose of the Study:

To review and compare six common fluid pumping methods for microfluidic cell sorting.
To discuss the advantages and disadvantages of each pumping technique.
To highlight emerging methods and future trends in microfluidic cell sorting.

Main Methods:

Review of established pumping methods: syringe, peristaltic, gravity-driven, hydrostatic, centrifugal, and electroosmotic.
Analysis of their performance characteristics (e.g., flow rate, pulsatility, power requirements).
Discussion of their suitability for microfluidic cell sorting applications.

Main Results:

Syringe pumps offer accessibility but lack portability and can have flow issues.
Peristaltic pumps allow reversible flow but introduce pulsatility.
Gravity/hydrostatic methods are simple but low throughput; centrifugal requires external hardware; electroosmotic needs high voltages.
Emerging methods show promise for advanced applications.

Conclusions:

Each pumping method has specific trade-offs for microfluidic cell sorting.
The choice of pump depends on application requirements like throughput, portability, and cell viability.
Future microfluidic cell sorting will likely feature highly integrated, high-throughput, low-volume systems.