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Published on: April 23, 2012
Improvement of Targeted Fungi Secondary Metabolite Production Using a Systematic Experimental Design and Chemometrics
Lamonielli F Michaliski1, Laura P Ióca1, Leandro S Oliveira1
1Instituto de Química de São Carlos, Universidade de São Paulo, CP 780, São Carlos CEP 13560-970, SP, Brazil.
Abstract:
Fungi are well-known producers of chemically diverse and biologically active secondary metabolites. However, their production yields through fermentation may hamper structural analysis and biological activity downstream investigations. Herein, a systematic experimental design that varies multiple cultivation parameters, followed by chemometrics analysis on HPLC-UV-MS or UHPLC-HRMS/MS data, is presented to enhance the production yield of fungal natural products. The overall procedure typically requires 3-4 months of work when first developed, and up to 3 months as a routine procedure.
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