Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Biosynthesis in Bacteria01:24

Biosynthesis in Bacteria

32
Biosynthesis in bacteria is a fundamental anabolic process that generates essential macromolecules, including proteins, nucleic acids, lipids, and polysaccharides. These macromolecules are critical for cellular growth, replication, and function. The process is tightly regulated and energetically linked to catabolic pathways to ensure optimal resource utilization.Biosynthetic pathways begin with precursor metabolites such as pyruvate, acetyl-CoA, and glucose-6-phosphate derived from glycolysis,...
32
Stringent Response in E. coli01:23

Stringent Response in E. coli

28
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
28

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Engineering a Synthesis Platform of Galactosylglycerol Based on Whole Cells with Surface-Displayed β-Galactosidase via Transglycosylation Catalysis and Glucose Consumption.

Journal of agricultural and food chemistry·2026
Same author

Enhanced Synthesis Ability of a β-1,3-Galactosyltransferase from <i>Proteus mirabilis</i> with Mechanistic Dissection of Transglycosylation.

Journal of agricultural and food chemistry·2026
Same author

Engineering the Bridge-like Structure of Esterase Est7 Enhanced the Synthesis of Eicosapentaenoic Acid Monoglyceride by Expanding Substrate Access and Modifying Fatty Acid Selectivity.

Journal of agricultural and food chemistry·2025
Same author

Efficient Bioconversion of Crystalline α-Chitin to <i>N</i>-Acetyl-D-glucosamine Driven by a Novel β-<i>N</i>-Acetylglucosaminidase and the Promotion Mechanism Analysis.

Journal of agricultural and food chemistry·2025
Same author

Biocatalytic Preparation of High-Purity DHA-Enriched Partial Glycerides through Semirational Design and Modification of Lipase Lip1897.

Journal of agricultural and food chemistry·2025
Same author

In Silico Enzymolysis-Guided Mining of Aminopeptidases with Molecular Insights into Their Substrate Specificity Mechanism.

Journal of agricultural and food chemistry·2024
Same journal

Removal of Codispersible Residual Impurities from CuInS<sub>2</sub>/ZnS Quantum Dots for Window-Replaceable Luminescent Solar Concentrators.

ACS applied materials & interfaces·2026
Same journal

Durable Core-Shell Scatterer Coating with Heat Storage for Radiative Cooling.

ACS applied materials & interfaces·2026
Same journal

Calix[6]arene-Based Interlocked Inverse Vulcanizate Enabling Network-Interface Cooperative Reinforcement in Natural Rubber/Carbon Black Composites.

ACS applied materials & interfaces·2026
Same journal

Resolving Thermal Accumulation and Rigid-Soft Interface Mismatch in Stretchable Electronics with Cubic Boron Nitride Composite Islands.

ACS applied materials & interfaces·2026
Same journal

Enhancing Conversion Reversibility and Initial Coulombic Efficiency of SnO<sub>2</sub> Anodes via NiO/Ni-Carbon Interfacial Design.

ACS applied materials & interfaces·2026
Same journal

Multidimensional Interface Structure Design for High-Efficiency Optically Controlled Semiconductor Devices: A Case Study on Memristive Synapses.

ACS applied materials & interfaces·2026
See all related articles

Related Experiment Video

Updated: Jul 16, 2025

Escherichia coli-Based Cell-Free Protein Synthesis: Protocols for a robust, flexible, and accessible platform technology
09:45

Escherichia coli-Based Cell-Free Protein Synthesis: Protocols for a robust, flexible, and accessible platform technology

Published on: February 25, 2019

35.2K

Engineered Escherichia coli Consortia Function in a Programmable Pattern for Multiple Enzymatic Biosynthesis.

Wenxue Zhang1, Hao Dong1,2, Xiaoli Wang1

  • 1State Key Laboratory of Bioreactor Engineering, School of Biotechnology, East China University of Science and Technology, Shanghai 200237, China.

ACS Applied Materials & Interfaces
|September 22, 2023
PubMed
Summary
This summary is machine-generated.

This study introduces a programmable cell assembly method for microbial consortia, enhancing biocatalysis efficiency for complex synthetic pathways like d-phenyllactic acid (d-PLA) production.

Keywords:
engineered biofilmenzyme coordinationmicrobial consortiarational designsubstrate channeling

More Related Videos

Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials
10:28

Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials

Published on: March 9, 2017

9.0K
Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX
10:09

Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX

Published on: June 27, 2017

13.4K

Related Experiment Videos

Last Updated: Jul 16, 2025

Escherichia coli-Based Cell-Free Protein Synthesis: Protocols for a robust, flexible, and accessible platform technology
09:45

Escherichia coli-Based Cell-Free Protein Synthesis: Protocols for a robust, flexible, and accessible platform technology

Published on: February 25, 2019

35.2K
Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials
10:28

Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials

Published on: March 9, 2017

9.0K
Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX
10:09

Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX

Published on: June 27, 2017

13.4K

Area of Science:

  • Synthetic biology
  • Biotechnology
  • Metabolic engineering

Background:

  • Coordinating microbial consortia for complex synthetic pathways is crucial for biomanufacturing.
  • Current methods face challenges in optimizing catalytic efficiency and enzyme arrangement.

Purpose of the Study:

  • To develop a programmable method for assembling living cells using surface display of affinity groups.
  • To optimize whole-cell catalysis through rational arrangement of cell assemblies and enzymes.
  • To enhance the production of d-phenyllactic acid (d-PLA) using engineered microbial consortia.

Main Methods:

  • Utilized surface display of affinity groups for programmable cell assembly.
  • Rationally arranged four enzymes for d-PLA synthesis, considering substrate channeling and protein expression.
  • Engineered microbial consortia for whole-cell catalysis.

Main Results:

  • Achieved 1.31-fold and 2.55-fold higher d-PLA production efficiencies compared to biofilm and whole-cell catalysts, respectively.
  • Demonstrated substrate channeling between co-immobilized rate-limiting enzymes, improving catalytic efficiency by 3.67-fold.
  • Obtained a maximum d-PLA yield of 102.85 ± 3.39 mM from 140 mM benzaldehyde.

Conclusions:

  • The developed cell assembly method enables coordinated microbial consortia for efficient multi-enzyme biosynthesis.
  • This approach offers a novel strategy for optimizing biocatalysis in engineered microbial systems.
  • The study advances the field of biomanufacturing through improved catalytic efficiency and product yield.