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A dual-functional single-atom Fe nanozyme-based sensitive colorimetric sensor for tannins quantification in brandy.

Xinrou Wang¹, Huan Liu², Cailin Qiao¹

¹Key Laboratory for Biorheological Science and Technology of Ministry of Education, Bioengineering College of Chongqing University, Chongqing 400044, PR China.

|September 24, 2023

Summary

A new colorimetric sensor using single-atom iron nanozyme (Fe@CN-20) offers a rapid and sensitive method for detecting tannins. This approach simplifies analysis and provides clear visualization, overcoming limitations of traditional techniques.

Keywords:

Single atom nanozyme Tannic acid UV colorimetric

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Area of Science:

Analytical Chemistry
Materials Science
Biotechnology

Background:

Traditional tannins detection methods are complex, time-consuming, and lack sensitivity.
Modern techniques like liquid chromatography are expertise-intensive and have poor data visualization.
A simplified, rapid, and visualized detection method for tannins is needed.

Purpose of the Study:

To develop a novel colorimetric sensor for efficient tannins detection.
To utilize a single-atom Fe nanozyme (Fe@CN-20) for enhanced detection capabilities.
To provide a simplified and visualized alternative to existing tannins analysis.

Main Methods:

Development of a colorimetric sensor based on single-atom Fe nanozyme (Fe@CN-20).
Utilizing Fe@CN-20's laccase-like and oxidase-like activities for simultaneous oxidation of tannins and TMB substrate.
Employing a competitive reaction between tannins and TMB for quantification.

Main Results:

The Fe@CN-20/TMB system achieved a detection range of 5-100 mg/L for tannic acid.
A low detection limit of 0.13 mg/L (S/N = 3) was established.
Analysis time was reduced to approximately 30 minutes with successful application in brandy samples.

Conclusions:

The developed Fe@CN-20 sensor is simple, sensitive, and rapid for tannins detection.
The sensor provides strong visualization, addressing a key limitation of other methods.
This novel approach offers a promising alternative for accurate and efficient tannins quantification.