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Related Experiment Video

Updated: Jul 15, 2025

Measuring Carbon Content in Airway Macrophages Exposed to Carbon-Containing Particulate Matters
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Lysosomal dysfunction in carbon black-induced lung disorders.

Mingtong Gao1, Xiao Ge2, Yun Li1

  • 1School of Pharmacy, Nanjing University of Chinese Medicine, 138 Xianlin Road, Qixia District, Nanjing, Jiangsu 210023, China.

The Science of the Total Environment
|September 24, 2023
PubMed
Summary

Carbon black (CB) particles cause lung damage by affecting lysosomes. Alkalizing lysosomes or using neferine from Nelumbinis plumula may protect against these pollution-related lung disorders.

Keywords:
Carbon blackInflammationLung disorderLysosomeNeferine

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Area of Science:

  • Environmental Health
  • Toxicology
  • Cell Biology

Background:

  • Carbon black (CB) is a major component of particulate pollution.
  • CB exposure is linked to significant lung-related health issues.

Purpose of the Study:

  • Investigate the harmful effects of aggregated CB on lung tissues and cells.
  • Explore the mechanism of CB-induced lung damage, focusing on lysosomes.
  • Evaluate the protective potential of neferine against CB toxicity.

Main Methods:

  • In vitro and in vivo studies using lung tissues and alveolar epithelial cells.
  • Analysis of inflammation, necrosis, and fibrosis markers.
  • Assessment of cell viability, cell death, and reactive oxygen species generation.
  • Investigation of lysosomal membrane permeabilization and alkalization.

Main Results:

  • CB exposure induced inflammation, necrosis, and fibrosis in vivo.
  • In vitro, CB decreased cell viability, increased cell death and oxidative stress.
  • CB targeted lysosomes, causing membrane permeabilization.
  • Lysosomal alkalization reduced CB's harmful effects.
  • Neferine demonstrated protective effects against CB-induced lung damage.

Conclusions:

  • CB particles induce lung disorders through lysosomal damage.
  • Lysosomal function modulation offers a potential therapeutic strategy for CB-induced lung disease.
  • Neferine presents a promising therapeutic agent for pollution-related lung conditions.