Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Replication origins are attached to the nuclear skeleton.

S V Razin, M G Kekelidze, E M Lukanidin

    Nucleic Acids Research
    |October 24, 1986
    PubMed
    Summary

    Replication origins (oriDNA) in chicken cells are located within DNA fragments attached to the nuclear matrix. These origin sequences are found in erythrocyte nuclear matrix DNA, suggesting their stability during cell differentiation.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Achieving Maximum Efficiency of A549 Cell Transfection with a Plasmid Delivered by Polyethylenimine-Based Polyplexes.

    Doklady. Biochemistry and biophysics·2026
    Same author

    Modular Nanotransporters Containing Anti-PSMA Nanobodies Are Able to Penetrate into the Nuclei of Prostate Cancer Cells.

    Doklady. Biochemistry and biophysics·2026
    Same author

    Optimization of A549 Cell Transfection Efficiency with a Plasmid Encoding the N-Protein of the SARS-CoV-2 Virus.

    Doklady. Biochemistry and biophysics·2025
    Same author

    Modular Nanotransporters Containing Keap1 Monobodies Are Capable of Reducing the Toxic Effect of Acetaminophen on the Liver of Mice.

    Doklady. Biochemistry and biophysics·2025
    Same author

    Increasing the Accumulation of Modular Nanotransporters in Mouse Tumors by Attaching Polyethylene Glycol to These Nanotransporters with the Possibility of Its Release into the Tumors.

    Doklady. Biochemistry and biophysics·2025
    Same author

    GALA3-Containing Modular Nanotransporters Are Capable of Delivering Keap1 Monobody to Target Cells and Inhibiting the Formation of Reactive Oxygen Species in the Cells.

    Doklady. Biochemistry and biophysics·2025

    Area of Science:

    • Molecular Biology
    • Cell Biology
    • Genetics

    Background:

    • DNA replication origins (oriDNA) are crucial for initiating DNA synthesis.
    • Nuclear matrix DNA (nmDNA) plays a role in genome organization and replication.
    • Chicken erythroblasts provide a model for studying DNA replication and nuclear architecture.

    Purpose of the Study:

    • To isolate and characterize DNA fragments containing replication origins (oriDNA) from chicken erythroblasts.
    • To investigate the association of oriDNA with the nuclear matrix.
    • To map replication origins within the alpha-globine gene domain and their relation to DNA attachment sites.

    Main Methods:

    • Isolation of DNA fragments containing replication origins (oriDNA) using a modified Zannis-Hadjopoulos et al. procedure.

    Related Experiment Videos

  • Renaturation reactions using total DNA or nuclear matrix DNA (nmDNA) from erythroblasts and erythrocytes.
  • Hybridization experiments to map oriDNA positions within the alpha-globine gene domain.

    • Main Results:

    • Erythroblast nmDNA contains a specific subset of unique sequences, with 5 kb fragments representing a quarter of total unique sequences.
    • Erythrocyte nmDNA fragments (5 kb) constitute a smaller fraction of unique DNA and are fully contained within erythroblast nmDNA.
    • Virtually all oriDNA sequences are present in the erythrocyte nmDNA fraction.
    • Replication origins within the alpha-globine gene domain map to permanent DNA attachment sites on the nuclear matrix.

    Conclusions:

    • Replication origins are preferentially associated with the nuclear matrix in chicken erythroblasts.
    • The nuclear matrix of mature erythrocytes retains sequences corresponding to replication origins found in erythroblasts.
    • Replication origins coincide with sites of permanent DNA attachment to the nuclear matrix, suggesting a role in replication initiation and genome organization.