Topical dexpanthenol effects on physiological parameters of the stratum corneum by Confocal Raman Microspectroscopy

Area of Science:

• Dermatology
• Biochemistry
• Optical Physics

Background:

• Dexpanthenol is known for moisturizing and skin barrier repair.
• Its precise mechanisms of action remain under investigation.
• Confocal Raman Microspectroscopy offers non-invasive in vivo analysis of skin molecular changes.

Purpose of the Study:

• To investigate the effects of topical dexpanthenol on stratum corneum (SC) components.
• To evaluate physiological parameters within the SC after dexpanthenol application.
• To elucidate the molecular mechanisms underlying dexpanthenol's skin benefits.

Main Methods:

• Ten healthy female subjects participated in the study.
• Confocal Raman Spectrometer Skin Analyzer 3510 was used for skin evaluation.
• Spectral data were collected from the forearm before and 2 hours after applying a 5% dexpanthenol formulation.

Main Results:

• A significant decrease in natural moisturizing factor content was observed post-application.
• Increased hexagonal organization of intercellular lipids in the upper SC was noted.
• Enhanced β-pleated sheet conformation of keratin indicated altered protein structure.

Conclusions:

• Topical dexpanthenol increases the fluidity of SC lipid and protein components.
• These findings support dexpanthenol's role in maintaining skin hydration and preventing transepidermal water loss.
• The study provides insights into dexpanthenol's biochemical action mechanisms.