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Updated: Jul 15, 2025

Analysis of Protein-protein Interactions and Co-localization Between Components of Gap, Tight, and Adherens Junctions in Murine Mammary Glands
Published on: May 30, 2017
Using PrISMa to reveal the interactome of the human claudins family
Daniel Perez-Hernandez1, Lorena Suarez-Artiles2, Mattson S O Jones1
1Department of Infection and Immunity, Luxembourg Institute of Health, 1A Rue Thomas Edison, 1445 Strassen, Luxembourg.
Abstract:
Here, we provide a protocol for the systematic screening of protein-protein interactions mediated by short linear motifs using the Protein Interaction Screen on a peptide Matrix (PrISMa) technique. We describe how to pull down interacting proteins in a parallelized manner and identify them by mass spectrometry. Finally, we describe a bioinformatic workflow necessary to identify highly probable interaction partners in the large-scale dataset. We describe the application of this method for the transient interactome of the claudin protein family. For complete details on the use and execution of this protocol, please refer to Suarez-Artiles et al.1.
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