Tunable Double-Network GelMA/Alginate Hydrogels for Platelet Lysate-Derived Protein Delivery
Andrea Marfoglia1,2, Fahd Tibourtine1, Ludovic Pilloux2
1CIRIMAT, Université Toulouse 3 Paul Sabatier, Toulouse INP, CNRS, Université de Toulouse, 31062 Toulouse, France.
Bioengineering (Basel, Switzerland)
|September 28, 2023
Summary
This study characterizes GelMA/alginate hydrogels for tissue engineering. The developed bioactive materials show potential for cell studies and drug delivery applications.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Regenerative Medicine
Background:
- Hydrogels are versatile biomaterials for tissue engineering and drug delivery.
- GelMA/alginate hydrogels offer ECM-like properties.
- Human platelet lysate (PL) is a rich source of growth factors.
Purpose of the Study:
- To characterize GelMA/alginate hydrogels loaded with human platelet lysate (PL).
- To evaluate the physicochemical properties, protein transport, degradation, and biocompatibility of these hydrogels.
- To explore their potential as bioactive materials for cell studies.
Main Methods:
- Rheological characterization of GelMA/alginate gels.
- Physicochemical analysis including pore size and degradation rate.
- Evaluation of PL protein release kinetics.
- Biocompatibility assessment.
Main Results:
- Alginate incorporation shifted gel mechanics from elastic to viscoelastic via calcium crosslinking.
- Approximately 90% of loaded PL proteins were released.
- Gel degradation profile was correlated with protein release.
- The hydrogels demonstrated good biocompatibility.
Conclusions:
- GelMA/alginate hydrogels are tunable bioactive materials.
- These hydrogels effectively transport and release therapeutic proteins.
- The developed hydrogels are promising substrates for tissue engineering and cell-based applications.
