Related Experiment Video
Updated: Jun 28, 2026
12:31
A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
Published on: February 28, 2015
15.2K
Paper-Based Aptasensors: Working Principles, Detection Modes, and Applications
Anastasios Economou1, Christos Kokkinos1, Leda Bousiakou2
1Laboratory of Analytical Chemistry, Department of Chemistry, National and Kapodistrian University of Athens, 15771 Athens, Greece.
Sensors (Basel, Switzerland)
|September 28, 2023
Summary
This review explores paper-based aptasensors, which combine aptamers and paper devices for low-cost, on-site detection. These aptasensors offer a promising alternative to traditional methods for various analytes.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Materials Science
Background:
- Aptamers are highly specific oligonucleotide-based bioreceptors, offering advantages over antibodies in bioassays and biosensors.
- Paper-based analytical devices (PADs) provide a low-cost, portable platform for on-site and point-of-care diagnostics.
- Combining aptamers with PADs creates paper-based aptasensors, enhancing diagnostic capabilities.
Purpose of the Study:
- To provide a comprehensive overview of paper-based aptasensors.
- To discuss the fabrication, working principles, and detection modes of these devices.
- To highlight applications and future prospects in analyte detection.
Main Methods:
- Review of literature on paper-based aptasensor fabrication and design.
- Analysis of aptamer properties as biorecognition elements.
- Discussion of various detection strategies employed in aptasensing PADs.
Main Results:
- Paper-based aptasensors integrate aptamers with PADs for sensitive and specific detection.
- Diverse applications demonstrated for detecting ions, small molecules, proteins, and cells.
- The review covers fabrication methods, working principles, and detection modes.
Conclusions:
- Paper-based aptasensors represent a significant advancement in low-cost, portable diagnostic tools.
- These devices offer a viable alternative to traditional bioassays for point-of-care applications.
- Future research should address challenges and explore new prospects for enhanced performance and broader utility.
More Related Videos
Related Concept Videos
01:15Potentiometry: Membrane Electrodes
Membrane electrodes, also known as p-ion electrodes, use membranes that selectively interact with free analyte ions, generating a potential difference across the membrane. The resulting membrane potential, known as the asymmetry potential, is not zero even when analyte concentrations on both sides of the membrane are equal. The membrane's response is typically not selective to a single analyte but proportional to the concentration of all ions in the sample solution capable of interacting at the...
01:10Amperometry: Overview
Amperometry is a technique commonly used to measure the concentration of specific analytes in a solution by monitoring the electric current generated during an electrochemical reaction. It involves applying a constant potential between a working electrode and a reference electrode to measure the resulting current, which is proportional to the concentration of the analyte. The Clark oxygen electrode operates based on this principle of amperometry. It consists of a cathode and an anode enclosed...
01:17Microbial Biosensors
Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...