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Long non-coding RNAs as potential biomarkers or therapeutic targets in gastric cancer

Nahid Askari1, Behnaz Salek Esfahani2, Sepideh Parvizpour3,4

  • 1Department of Biotechnology, Institute of Sciences and High Technology and Environmental Sciences, Graduate University of Advanced Technology, End of Haft Bagh-e-Alavi Highway, Kerman, Iran.

Gastroenterology and Hepatology From Bed to Bench
|September 28, 2023
PubMed
Abstract

Insights

This study identified key long non-coding RNAs (lncRNAs) and messenger RNAs (mRNAs) in gastric cancer. These findings offer potential new targets for anti-cancer therapies and diagnostic biomarkers.

Area of Science:

  • Oncology
  • Genetics
  • Molecular Biology

Background:

  • Gastric cancer (GC) is a leading cause of cancer death globally.
  • Disordered expression of non-coding RNAs (ncRNAs), particularly long non-coding RNAs (lncRNAs), is implicated in GC progression, invasion, and metastasis.
  • lncRNAs play a crucial role in regulating gene expression and advancing GC development.

Purpose of the Study:

  • To identify differentially expressed lncRNAs (DElncRNAs) and messenger RNAs (DEmRNAs) in gastric cancer by integrating multiple microarray datasets.
  • To discover potential therapeutic targets and diagnostic biomarkers for gastric cancer through meta-analysis.
  • To elucidate the regulatory roles of lncRNAs in gastric cancer pathogenesis.

Main Methods:

  • Meta-analysis of three gastric cancer tissue microarray datasets to identify DElncRNAs and DEmRNAs using the "Limma" package.
  • Utilized the RNAInter database to predict interactions between DElncRNAs and DEmRNAs.
  • Performed functional enrichment analysis and gene ontology analysis on DEmRNAs using ClusterProfiler and GOplot.

Main Results:

  • Identified 9 DElncRNAs (5 up-regulated, 4 down-regulated) and 856 DEmRNAs (451 up-regulated, 405 down-regulated) between tumor and normal gastric tissues.
  • Predicted 117 DEmRNAs as interactors of six key DElncRNAs: H19, WT1-AS, EMX2OS, HOTAIR, ZEB1-AS1, and LINC00261.
  • Established a network of drug-gene interactions for the identified genes.

Conclusions:

  • The study identified novel DElncRNAs and DEmRNAs in gastric cancer, providing insights into carcinogenesis.
  • These findings highlight potential biomarkers for gastric cancer prognosis.
  • The projected network of drug-gene interactions offers promising avenues for developing novel anti-cancer therapeutics for gastric cancer.
Keywords:
BiomarkerGastric cancerLong non-coding RNAs (lncRNAs)Messenger RNA (mRNA)Microarray analysis

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